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Quantification of Cable Bacteria in Marine Sediments via qPCR
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Cable bacteria (Deltaproteobacteria, Desulfobulbaceae) are long filamentous sulfur-oxidizing bacteria that generate long-distance electric currents running through the bacterial filaments. This way, they couple the oxidation of sulfide in deeper sediment layers to the reduction of oxygen or nitrate near the sediment-water interface. Cable bacteria are found in a wide range of aquatic sediments, but an accurate procedure to assess their abundance is lacking. We developed a qPCR approach that quantifies cable bacteria in relation to other bacteria within the family Desulfobulbaceae. Primer sets targeting cable bacteria, Desulfobulbaceae and the total bacterial community were applied in qPCR with DNA extracted from marine sediment incubations. Amplicon sequencing of the 16S rRNA gene V4 region confirmed that cable bacteria were accurately enumerated by qPCR, and suggested novel diversity of cable bacteria. The conjoint quantification of current densities and cell densities revealed that individual filaments carry a mean current of ~110 pA and have a cell specific oxygen consumption rate of 69 fmol O2 cell-1 day-1. Overall, the qPCR method enables a better quantitative assessment of cable bacteria abundance, providing new metabolic insights at filament and cell level, and improving our understanding of the microbial ecology of electrogenic sediments.
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No RBINS Staff publications
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Quantifying sediment plumes induced by human activities by using MBES and SBES water column data combined with in situ measurement and water sampling: Feasible?
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RBINS Staff Publications 2021
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Quelques aspects des pratiques funéraires au Néolithique proche-oriental : la gestion de l’espace à Çatalhöyük
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Au Proche-Orient, des sépultures appartenant à des hommes, des femmes et des individus immatures, ont été retrouvées sous le sol des maisons néolithiques en Anatolie Centrale, au Levant Nord, au Levant Sud, et dans la Djezirah Iraquienne. Au sein de ses sépultures, les individus reposaient individuellement ou à plusieurs (simultanément ou successivement) dans une position évoquant celle d’un fœtus au sein de l’utérus. Les études sur leur contexte domestique et sur leur organisation spatiale sont des principales clefs pour l’interprétation des idées religieuses derrière ces pratiques. Dans le présent article, nous allons présenter quelques aspects des pratiques funéraires au Néolithique proche-oriental qui concernent surtout la gestion de l’espace funéraire à Çatalhöyük.
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ANTHROPOLOGICA ET PREHISTORICA
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Bibliographic references
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Quévy/Givry et Havay : fouille d’un fossé circulaire
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RBINS Staff Publications 2016
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QWIP: A Quantitative Metric for Quality Control of Aquatic Reflectance Spectral Shape Using the Apparent Visible Wavelength
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The colors of the ocean and inland waters span clear blue to turbid brown, and the corresponding spectral shapes of the water-leaving signal are diverse depending on the various types and concentrations of phytoplankton, sediment, detritus and colored dissolved organic matter. Here we present a simple metric developed from a global dataset spanning blue, green and brown water types to assess the quality of a measured or derived aquatic spectrum. The Quality Water Index Polynomial (QWIP) is founded on the Apparent Visible Wavelength (AVW), a one-dimensional geophysical metric of color that is inherently correlated to spectral shape calculated as a weighted harmonic mean across visible wavelengths. The QWIP represents a polynomial relationship between the hyperspectral AVW and a Normalized Difference Index (NDI) using red and green wavelengths. The QWIP score represents the difference between a spectrum’s AVW and NDI and the QWIP polynomial. The approach is tested extensively with both raw and quality controlled field data to identify spectra that fall outside the general trends observed in aquatic optics. For example, QWIP scores less than or greater than 0.2 would fail an initial screening and be subject to additional quality control. Common outliers tend to have spectral features related to: 1) incorrect removal of surface reflected skylight or 2) optically shallow water. The approach was applied to hyperspectral imagery from the Hyperspectral Imager for the Coastal Ocean (HICO), as well as to multispectral imagery from the Visual Infrared Imaging Radiometer Suite (VIIRS) using sensor-specific extrapolations to approximate AVW. This simple approach can be rapidly implemented in ocean color processing chains to provide a level of uncertainty about a measured or retrieved spectrum and flag questionable or unusual spectra for further analysis.
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RBINS Staff Publications 2022
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Radial porosity profiles: a new bone histological method for comparative developmental analysis of diametric limb bone growth
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RBINS Staff Publications 2022
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Radiocarbon chronology for the Early Gravettian of northern Europe: new AMS determinations for Maisières-Canal, Belgium
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RBINS Staff Publications 2016
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Rapid Local Adaptations in an Invasive Frog (Xenopus laevis): the Importance of Functional Trait Measurements to Predict Future Invasions
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RBINS Staff Publications 2020
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RBINS Staff Publications 2023 OA
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Recent advances in heteromorph ammonoid palaeobiology
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RBINS Staff Publications 2021