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Article Reference Molecular methods for the detection and identification of parasitoids within larval wheat midges
Three species of cecidomyiid midges (Diptera: Cecidomyiidae) cause significant yield losses on wheat in Europe: Sitodiplosis mosellana (Géhin), Contarinia tritici (Kirby) and Haplodiplosis marginata (von Roser). Eggs and young larvae may be parasitised by a complex of hymenopteran parasitoids belonging to the Pteromalidae and Platygastridae families which contributes to natural pest control. We have developed molecular tools for detecting and identifying seven parasitoid species previously encountered in Belgium inside individual wheat midge larvae. Barcode DNA sequences from COI, 18S and 28S genes were obtained from the midges and parasitoid species. Each of the three genes allowed all the species to be distinguished although 18S was the only one displaying a barcoding gap, both between parasitoids and midges, and at the species level. Based on the 18S gene, we developed a TaqMan assay to assess parasitism in midge larvae, regardless of the midge and parasitoid species. Next, two group-specific PCR primer pairs were generated, allowing the separate amplification of midge DNA or parasitoid DNA in parasitised individuals and subsequent identification by Sanger sequencing. Finally, species-specific primers were designed to identify six parasitoid species by simple PCR amplification. These tools were successfully applied to assess the parasitism rate of S. mosellana larvae in seven Belgian fields.
Located in Library / RBINS Staff Publications 2024
Inproceedings Reference Characterization of a multiple burial context from Pachacamac, Peru: complementarity between bioarchaeology and molecular archaeology
Pachacamac is a major precolumbian site located on Peru’s Central Coast. Covering approximately 6 km2, the site was occupied for over a thousand years before the Spanish conquest in the early sixteenth century. In 2012, the Ychsma Project discovered a unique Late Intermediate period (AD 900–1470) multiple burial (“Cx4”) made of two funerary chambers with a vegetal roof structure, containing over 110 intact and fragmentary deceased together with numerous grave goods. More than 60% of the individuals are subadults whose sex cannot be assigned using osteological observation. Among the adults, 23 females and 20 males were identified, and the sex of the remaining four individuals couldn’t be assigned with certainty. We aim to fully understand the sociobiology of the Cx4 population, including biological sex, using a combined bioarchaeology and molecular archaeology approach. Despite significant human modern contamination and low amounts of endogenous ancient DNA, our results show that sex could be assigned genetically in >70% of the cases, including subadults. Sex identification of infants, children and adolescents is crucial to fully understand this complex context and its funerary recruitment, and to perform an integrated and holistic analysis of all associated data.
Located in Library / RBINS Staff Publications 2024
Inproceedings Reference Paleo-genetic and paleo-pathological studies at Pachacamac: methodological issues and characterization of a multiple burial context (in Spanish)
Pachacamac is a major pre-Columbian site located on Peru’s Central Coast. Covering approximately 6 km2, the site was occupied for over a thousand years before the Spanish conquest in the early 16th century. In 2012, the Ychsma Project excavated a Late Intermediate Period (900 to 1470 AD) multiple tomb (Cx4) made of two funerary chambers covered by a vegetal roof, containing 89 deceased together with numerous grave goods. Over 60% of the individuals are subadults whose sex cannot be assigned based on osteological observations. Ancient DNA (aDNA) analysis can be useful for sex determination, general mitochondrial lineage (haplogroup) and disease diagnosis. However, non-endogenous DNA contamination of archaeological material is a recurrent problematic, since excavation, handling and storage usually don't fit with the precautions recommended for ancient DNA analysis. Nevertheless, we obtained aDNA results from several human individuals recovered from the Cx4 context. Our objectives are to complete the demographic information and to characterize the health status of the population buried in this pilgrimage site dedicated to the eponymous healing god. Despite significant human modern contamination and low amounts of endogenous ancient DNA, our results show that sex could be assigned genetically in >80% of the cases, including subadults. Sex identification of infants, children and adolescents is crucial to fully understand this complex context and its funerary recruitment, and to perform an integrated and holistic analysis of all associated data. The genetic data were also used to estimate the presence of several pathogens using the KrakenUniq taxonomical classification tool.
Located in Library / RBINS Staff Publications 2024
Article Reference Review of Trictenotomid beetles (Coleoptera: Trictenotomidae) of India and Sri Lanka
Located in Library / RBINS Staff Publications 2023 OA
Article Reference Shallow Suberitida (Porifera, Demospongiae) from Peru
Located in Library / RBINS Staff Publications 2023 OA
Article Reference Megastomia crovatoi Nofroni, Renda & Vannozzi, 2022 (Gastropoda: Pyramidellidae) alive in Bozcaada Island (Aegean Sea - Turkey)
Located in Library / RBINS Staff Publications 2023 OA
Article Reference Environmental and climatic inferences for Marine Isotope Stage 2 of southern Belgium (Meuse valley, Namur Province) based on rodent assemblages
The environmental and climatic conditions of the Late Pleistocene of Southern Belgium are here determined for the final part of Marine Isotope Stage 3 (MIS 3) and for MIS 2 on the basis of a study of rodent assemblages. This paper provides a synthesis of several sets of environmental and climatic data from Late Pleistocene sites, all of which are located in southern Belgium. One has previously been published (Caverne Marie-Jeanne), and seven are unpublished (Cavernes de Goyet, Trou des Nutons, Trou du Frontal, Trou de Chaleux, Grotte la Chefalize, Trou du Chˆene, and Trou du Sureau). The habitat weighting and quantified ecology methods are applied to rodent material housed in the Royal Belgian Institute of Natural Sciences (RBINS, Brussels), and previous radiocarbon dates are updated, in order to reconstruct past environments. Among all the sites under analysis, the quantified ecology method shows that Trou de Chaleux corresponds to the coldest temperatures and lowest precipitation. Trou de Chaleux, with a chronology between ca. 15,964–14,014 cal yr B.P., could probably be placed in Greenland Stadial 2 (GS2) or Heinrich Event 1 (HE1). It has a rodent assemblage associated with a predominance of open dry and rocky formations, the most abundant species being the collared lemming and the narrow-headed vole. These data are found to coincide with previous studies carried out on the large-mammal, herpetofaunal, and avifaunal associations of the site, as well as on small-mammal associations from other sites in southern Belgium with similar chronology, such as Grotte Walou. Taken together, this indicates that these latest Pleistocene intervals in southern Belgium were characterized by harsh climatic and environmental conditions. In contrast, the other assemblages under study yielded much more heterogeneous results, frequently inconsistent with an attribution to the Pleistocene. This is likely to be a result of their admixture with Holocene material due to recent intrusions.
Located in Library / RBINS Staff Publications 2023
Article Reference Een midden-Romeinse rurale site in de Sigma zone ‘Wijmeers 2’ (gemeente Wichelen, Oost-Vlaanderen)
Located in Library / RBINS Staff Publications 2024
Preprint Reference Somewhere I belong: phylogeny and morphological evolution in a species-rich lineage of ectoparasitic flatworms infecting cichlid fishes
Located in Library / RBINS Staff Publications 2021 OA
Inproceedings Reference DIVERSE ARCHIVAL HIV-1 GENOMES FROM THE 1980’S IN DR CONGO
Located in Library / RBINS Staff Publications 2023 OA