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Résumé : Un ensemble de restes fauniques découverts à Dave (province de Namur, Belgique), à proximité d’un dépôt de quatre haches néolithiques, n’y est pas directement associé. Les ossements animaux n’ont pas été déposés par l’Homme mais plus probablement accumulés par un rapace nocturne, le Grand-duc d’Europe. En outre, leur date de dépôt est vraisemblablement postérieure à celle des grandes lames de haches de Dave. Mots-clefs : rochers de Dave, commune et province de Namur (B), faune, oiseaux sauvages, Grand-duc d’Europe, Spatule blanche, Harle bièvre, mammifères sauvages, mammifères domestiques, datation radiométrique, haches de Dave. Abstract : Faunal remains have been discovered at Dave (Namur Province, Belgium) close to four Neolithic stone axes, but the two assemblages appear not to be directly associated. The animal bones do not reflect human activities but were most likely accumulated by an owl, the Eurasian Eagle-Owl. In addition, the faunal assemblage is presumably more recent than the big blades of axes from Dave. Keywords : Dave rocks, prov. of Namur (B), faunal remains, wild birds, Eurasian Eagle-Owl, Eurasian Spoonbill, Goosander, wild mammals, domestic mammals, radiometric dating, Dave axes.

In order to reach greenhouse gas emission reduction targets, atmospheric CO2 emissions from all industrial sectors need to be avoided. Globally, the cement production industry emits 2.4 Gt CO2 per year, or 7% of all CO2 emissions (IEA). While about a third of this could be reduced by using renewable energy sources, the remainder are process emissions from the calcination process. Lime or CaO is produced by heating limestone (CaCO3), emitting CO2. The Australian company Calix has developed a direct separation technology for capturing these process emissions; a pilot-scale installation is operational at the Lixhe cement plant in Belgium (Figure 1). The EU H2020-funded LEILAC2 project (Low Emission Intensity Lime and Cement 2: Demonstration Scale) upscaling and integrating a novel type of carbon capture technology. This technology aims to capture, at low cost, unavoidable process emissions from cement and lime plant. This large-scale capture plant will be installed at the Heidelberg Cement’s plant in Hannover, Germany, capturing 20% of a typical cement plant’s CO2 emission. Apart from the physical installation and operation of the capture unit, a business case will be developed for the downstream components of transport, use and geological storage for the captured CO2. In order to develop a business case, a very large number of options, combinations and scenarios for each these components need to be evaluated, taking into account the intricacies of for example dealing with geological data in economic calculations. The PSS suite of geo-techno-economic simulators has been developed by the Geological Survey of Belgium, specifically for creating forecasts on the deployment of CO2 capture and geological storage (CCS) technologies (Welkenhuysen et al., 2013). In PSS, investment decisions for the full CCS chain are simulated as a forecast in a non-deterministic way, considering uncertainty and flexibility. Especially for matching storage, these elements are essential. While capture in this demonstration project is a given, several scenarios will be analyzed: the current demo-scale, full-scale capture, and CO2-network integration. Due to its location, several CO2 transport options can be considered at the Hannover plant: from low-volume truck, railway or barge transport, up to ships and pipelines. Special attention is given to possible connections with ongoing and planned initiatives for infrastructure and hub development such as the Porthos project in the port of Rotterdam or the Northern Lights project offshore Norway. In the wider area around the capture location, North-Western Europe including the North Sea offshore area, there are many potential storage options available. Offshore storage options will be the primary targets for assessment, with many (nearly) depleted hydrocarbon fields and saline aquifers that are present in the southern North Sea. Storage aspects are treated as stochastic parameters, with for example storage capacity and injectivity of the reservoirs represented by probability density functions. In order to compare storage options, the degree of knowledge, uncertainty and economic and practical development feasibility of such a storage location needs to be assessed. An analysis of such storage classification systems is created by Tovar et al. (this conference). With the above-mentioned PSS method and CCS project development options, source-sink matching is performed to create forecasts on project and network development. Results will provide insight in the probability of preferred storage option development for steering exploration and development efforts, preferred transport modes and routes, the optimal timing of investments, and the influence of market parameters, such as the ETS price of CO2 emissions. Acknowledgments This research is carried out under the LEILAC2 project, which receives funding by the European Union’s Horizon 2020 research and innovation program under grant agreement number 884170. The LEILAC2 consortium consists of: Calix Europe SARL, HeidelbergCement AG, Ingenieurbüro Kühlerbau Neustad GmbH (IKN), Centre for Research and Technology Hellas (CERTH), Bundesanstalt für Geowissenschaften und Rohstoffe (BGR), Politecnico di Milano (POLIMI), Geological Survey of Belgium (RBINS-GSB), ENGIE Laborelec, Port of Rotterdam, Calix Limited, CIMPOR-Indústria de Cimentos SA and Lhoist Recherche et Development SA. References IEA, 2020. Energy Technology Perspectives 2020. https://www.iea.org/reports/energy-technology-perspectives-2020 Tovar, A., Piessens, K. & Welkenhuysen, K., this conference. Ranking CO2 storage capacities and identifying their technical, economic and regulatory constraints: A review of methods and screening criteria. Welkenhuysen K., Ramírez A., Swennen R. & Piessens K., 2013. Strategy for ranking potential CO2 storage reservoirs: A case study for Belgium. International Journal of Greenhouse Gas Control, 17, 431-449. http://dx.doi.org/10.1016/j.ijggc.2013.05.025

Most of today’s domesticates began as farm animals, but cat domestication took a different path. Cats became commensal of humans somewhere in the Fertile Crescent, attracted to early farmers’ settlements by rodent pests. Cat remains from Poland dated to 4,200 to 2,300 y BCE are currently the earliest evidence for the migration of the Near Eastern wildcat to Central Europe. Tracking the possible synanthropic origin of that migration, we used stable isotopes to investigate the paleodiet. We found that the ecological balance was already changed due to the expansion of Neolithic farmlands. We conclude that among the Late Neolithic Near Eastern wildcats from Poland were free-living individuals, who preyed on rodent pests and shared ecological niches with native European wildcats.Cat remains from Poland dated to 4,200 to 2,300 y BCE are currently the earliest evidence for the migration of the Near Eastern cat (NE cat), the ancestor of domestic cats, into Central Europe. This early immigration preceded the known establishment of housecat populations in the region by around 3,000 y. One hypothesis assumed that NE cats followed the migration of early farmers as synanthropes. In this study, we analyze the stable isotopes in six samples of Late Neolithic NE cat bones and further 34 of the associated fauna, including the European wildcat. We approximate the diet and trophic ecology of Late Neolithic felids in a broad context of contemporary wild and domestic animals and humans. In addition, we compared the ecology of Late Neolithic NE cats with the earliest domestic cats known from the territory of Poland, dating to the Roman Period. Our results reveal that human agricultural activity during the Late Neolithic had already impacted the isotopic signature of rodents in the ecosystem. These synanthropic pests constituted a significant proportion of the NE cat’s diet. Our interpretation is that Late Neolithic NE cats were opportunistic synanthropes, most probably free-living individuals (i.e., not directly relying on a human food supply). We explore niche partitioning between studied NE cats and the contemporary native European wildcats. We find only minor differences between the isotopic ecology of both these taxa. We conclude that, after the appearance of the NE cat, both felid taxa shared the ecological niches.

Anthropogenic factors have impacted the diversity and evolutionary trajectory of various species. This can be through factors such as pressure on population size or range, habitat fragmentation, or extensive manipulation and translocation. Here we use time-calibrated data to better understand the pattern and processes of evolution in the heavily manipulated European fallow deer (Dama dama). During the Pleistocene, fallow deer had a broad distribution across Europe and were found as far north as Britain during the Eemian interglacial. The last glacial period saw fallow deer retreat to southern refugia and they did not disperse north afterwards. Their recolonisation was mediated by people and, from northern Europe and the British Isles, fallow deer were transported around the world. We use ancient and modern mitochondrial DNA (mtDNA) and mitogenomic data from Eemian Britain to assess the pattern of change in distribution and lineage structure across Europe over time. We find founder effects and mixed lineages in the northern populations, and stability over time for populations in southern Europe. The Eemian sample was most similar to a lineage currently in Italy, suggesting an early establishment of the relevant refuge. We consider the implications for the integration of anthropogenic and natural processes towards a better understanding of the evolution of fallow deer in Europe.

Grey wolves (Canis lupus) are one of the few large terrestrial carnivores that have maintained a wide geographical distribution across the Northern Hemisphere throughout the Pleistocene and Holocene. Recent genetic studies have suggested that, despite this continuous presence, major demographic changes occurred in wolf populations between the Late Pleistocene and early Holocene, and that extant wolves trace their ancestry to a single Late Pleistocene population. Both the geographical origin of this ancestral population and how it became widespread remain unknown. Here, we used a spatially and temporally explicit modelling framework to analyse a data set of 90 modern and 45 ancient mitochondrial wolf genomes from across the Northern Hemisphere, spanning the last 50,000 years. Our results suggest that contemporary wolf populations trace their ancestry to an expansion from Beringia at the end of the Last Glacial Maximum, and that this process was most likely driven by Late Pleistocene ecological fluctuations that occurred across the Northern Hemisphere. This study provides direct ancient genetic evidence that long‐range migration has played an important role in the population history of a large carnivore, and provides insight into how wolves survived the wave of megafaunal extinctions at the end of the last glaciation. Moreover, because Late Pleistocene grey wolves were the likely source from which all modern dogs trace their origins, the demographic history described in this study has fundamental implications for understanding the geographical origin of the dog.

Archaeological evidence indicates that pig domestication had begun by ∼10,500 y before the present (BP) in the Near East, and mitochondrial DNA (mtDNA) suggests that pigs arrived in Europe alongside farmers ∼8,500 y BP. A few thousand years after the introduction of Near Eastern pigs into Europe, however, their characteristic mtDNA signature disappeared and was replaced by haplotypes associated with European wild boars. This turnover could be accounted for by substantial gene flow from local European wild boars, although it is also possible that European wild boars were domesticated independently without any genetic contribution from the Near East. To test these hypotheses, we obtained mtDNA sequences from 2,099 modern and ancient pig samples and 63 nuclear ancient genomes from Near Eastern and European pigs. Our analyses revealed that European domestic pigs dating from 7,100 to 6,000 y BP possessed both Near Eastern and European nuclear ancestry, while later pigs possessed no more than 4\% Near Eastern ancestry, indicating that gene flow from European wild boars resulted in a near-complete disappearance of Near East ancestry. In addition, we demonstrate that a variant at a locus encoding black coat color likely originated in the Near East and persisted in European pigs. Altogether, our results indicate that while pigs were not independently domesticated in Europe, the vast majority of human-mediated selection over the past 5,000 y focused on the genomic fraction derived from the European wild boars, and not on the fraction that was selected by early Neolithic farmers over the first 2,500 y of the domestication process.

While sequencing ancient DNA (aDNA) from archaeological material is now commonplace, very few attempts to sequence ancient transcriptomes have been made, even from typically stable deposition environments such as permafrost. This is presumably due to assumptions that RNA completely degrades relatively quickly, particularly when dealing with autolytic, nuclease-rich mammalian tissues. However, given the recent successes in sequencing ancient RNA (aRNA) from various sources including plants and animals, we suspect that these assumptions may be incorrect or exaggerated. To challenge the underlying dogma, we generated shotgun RNA data from sources that might normally be dismissed for such study. Here, we present aRNA data generated from two historical wolf skins, and permafrost-preserved liver tissue of a 14,300-year-old Pleistocene canid. Not only is the latter the oldest RNA ever to be sequenced, but it also shows evidence of biologically relevant tissue specificity and close similarity to equivalent data derived from modern-day control tissue. Other hallmarks of RNA sequencing (RNA-seq) data such as exon-exon junction presence and high endogenous ribosomal RNA (rRNA) content confirms our data’s authenticity. By performing independent technical library replicates using two high-throughput sequencing platforms, we show not only that aRNA can survive for extended periods in mammalian tissues but also that it has potential for tissue identification. aRNA also has possible further potential, such as identifying in vivo genome activity and adaptation, when sequenced using this technology.