Echinoderms are marine water invertebrates that are represented by more than 7000 extant species, grouped in five classes and showing diverse morphologies (starfish, sea lilies, feather stars, sea urchins, sea cucumbers, brittle and basket stars). In an effort to further study their diversity, DNA barcodes (DNA fragments of the 5’ end of the cytochrome c oxidase subunit I gene, COI) have been used to complement morphological examination in identifying evolutionary lineages. Although divergent clusters of COI sequences were reported to generally match morphological species delineations, they also revealed some discrepancies, suggesting overlooked species, ecophenotypic variation or multiple COI lineages within one species. Here, we sequenced COI fragments of 312 shallow-water echinoderms of the East Coast of South Africa (KwaZulu-Natal Province) and compared morphological identifications with species delimitations obtained with four methods that are exclusively based on COI sequences. We identified a total of 103 morphospecies including 18 that did not exactly match described species. We also report 46 COI sequences that showed large divergences (>5% p-distances) with those available to date and publish the first COI sequences for 30 species. Our analyses also identified discordances between morphological identifications and COI-based species delimitations for a considerable proportion of the morphospecies studied here (49/103). For most of them, further investigation is necessary to keep a sound connection between taxonomy and the growing importance of DNA-based research.
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RBINS Staff Publications 2022
DNA BARCODING OF FIRE ANTS AND THIEF ANTS (GENUS SOLENOPSIS) OF THE ECUADORIAN ANDES AS A TOOL FOR BIODIVERSITY RESEARCH SONET, G. (1), Nagy, Z. T. (1), Jacquemin, J. (2), Wauters, N. (2), Delsinne, T. (2), Leponce, M. (2) (1) Joint Experimental Molecular Unit, Royal Belgian Institute of Natural Sciences, Brussels & Royal Museum for Central Africa, Tervuren, Belgium (2) Royal Belgian Institute of Natural Sciences & Université Libre de Bruxelles, Belgium Lightning Talk, Barcoding Insects 2, Napier 208, Friday, 15:00 to 15:07 Poster Location: B25 Members of the genus Solenopsis are among the most abundant ants in tropical rainforests. They are represented by more than 200 described species worldwide and some are dreadful invasive species. The identification to the species level is hampered by a dearth of diagnostic morphological characters and represents a serious limitative step in biodiversity inventories and in the study of invasive species. We set up and validated a DNA barcoding procedure to identify ants of the genus Solenopsis collected in the Podocarpus National Park of the Ecuadorian Andes. Complete specimens were used for DNA extraction and subsequently preserved as vouchers to allow further morphological analysis. More than 14 new molecular operational taxonomic units were identified by the standard DNA barcode fragment. In some cases specimens from a single morpho-species occurring at different altitudes could be distinguished. This study resulted in an appropriate laboratory protocol and a reference library useful to identify ants of the genus Solenopsis in the Ecuadorian Andes.
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