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Barcoding and traditional health practitioner perspectives are informative to monitor and conserve frogs and reptiles traded for traditional medicine in urban South Africa
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Abstract Previous literature suggests that Indigenous cultural practices, specifically traditional medicine, are commonplace among urban communities contrary to the general conception that such practices are restricted to rural societies. We reviewed previous literature for records of herptiles (frog and reptile species) sold by traditional health practitioners in urban South Africa, then used visual confirmation surveys, DNA barcoding and folk taxonomy to identify the herptile species that were on sale. Additionally, we interviewed 11 IsiZulu and SePedi speaking traditional health practitioners to document details of the collection and pricing of herptile specimens along with the practitioners' views of current conservation measures for traditional medicine markets. The 34 herptile species recorded in previous literature on traditional medicine markets included endangered and non-native species. Spectrophotometry measurements of the DNA we extracted from the tissue of herptiles used in traditional medicine were an unreliable predictor of whether those extractions would be suitable for further experimental work. From our initial set of 111 tissue samples, 81 sequencing reactions were successful and 55 of those sequences had species-level matches to COI reference sequences on the NCBI GenBank and/or BOLD databases. Molecular identification revealed that traditional health practitioners correctly labelled 77% of the samples that we successfully identified with DNA barcoding in this study. Our mixed methodology approach is useful for conservation planning as it updates knowledge of animal use in Indigenous remedies and can accurately identify species of high conservation priority. Furthermore, this study highlights the possibility of collaborative conservation planning with traditional health practitioners.
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RBINS Staff Publications 2025
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Bargaining a net gain compensation agreement between a marine renewable energy developer and a marine protected area manager.
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RBINS Staff Publications
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Barium
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RBINS Staff Publications 2024
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BASE – BAsicranial Sex Estimation: An R package for sexing adult western European individuals from the cranial base morphometry in bioarchaeology and forensic anthropology
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Sexing an adult human skeleton from its fragmented remains is a tremendous challenge in both archaeological and forensic contexts. Although reliable sex estimation methods using the os coxae do exist, as well as the possibility of performing a secondary sexual diagnosis within a funerary assemblage, it is essential in the case of fragmented individuals to set up an alternative sex estimation method based on a skeletal element that is both sexually dimorphic and has a high taphonomic survival rate. This study investigated the sexual dimorphism of the cranial base (occipital and temporal bones), through an exclusively metric approach, in 537 identified western European adults. Using logistic regressions, thirteen predictive models were built up, which yielded up to 86.8% accuracy after cross-validation and with a decision threshold of 0.70. BASE is an R package with a graphic user interface that can be used to apply these predictive models to a target adult individual (https://archive.softwareheritage.org/browse/directory/5e3e3753e1f9c360b0ddbebaa2d2b42e5d7686d9). This paper provides measurement definitions, practical details and user recommendations. BASE is a new sex estimation tool which is free of charge, fast and easy to use, and allows anthropologists to collect biological data from larger numbers of individuals, even when their skeletons are highly fragmented and damaged. This new method can certainly contribute to a better understanding of past populations and will be of considerable value to forensic investigations in today’s context of steadily rising numbers of missing persons. BOUCHERIE A. , POLET C. , MARTINE V., LEFÈVRE P., SANTOS F., 2025. BASE – BAsicranial Sex Estimation: An R package for sexing adult western European individuals from the cranial base morphometry in bioarchaeology and forensic anthropology. , (2) :
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RBINS Staff Publications 2025
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Baseline levels and trophic transfer of persistent organic pollutants in sediments and biota from the Congo River Basin (DR Congo)
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RBINS Staff Publications
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Bayerische Steine in den Niederlanden und Flandern
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RBINS Staff Publications 2019
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Bedforms as Benthic Habitats: Living
on the Edge, Chaos, Order and Complexity
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Bedforms as benthic habitats are studied increasingly as acquisition and analysis of acoustic data improve in capturing, visualizing and quantifying terrain variables on various scales. However, feedback mechanisms between geomorphology and benthos are not always clear and complexity increases where humans also affect the benthos-landscape relationship. Based on research-oriented seabed mapping along the Belgian part of the North Sea (BPNS), a synthesis is provided on where increased biodiversity has been observed in relation to active bedforms.
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RBINS Staff Publications 2017
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Beef, pork and mutton. An archaeological survey of the meat consumption in medieval and postmedieval towns in the southern Low Countries (Flanders & Brussels, Belgium)
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A survey is presented of archaeozoological information from medieval and postmedieval towns in the southern Low Countries (the present regions of Flanders and Brussels, in Belgium). Diachronic changes in the consumption of the three main domestic meat-suppliers (cattle, pig, sheep) in nine towns are investigated, and trends are compared among these towns. At the same time, possible geographical differences in meat consumption are traced. The observed differences in time and space are then explained as part of the economics of animal husbandry and of the interaction between town and countryside. From a methodological standpoint, this survey demonstrates that in a number of cases, information from archaeozoological contexts with varying depositional histories, often reflecting different socio-economic strata, can be combined to obtain a picture of meat consumption, and thus of the town's food provisioning, through time.
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RBINS Staff Publications 2017
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Behavior and body size modulate the defense of toxin‑containing sawfly larvae against ants
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RBINS Staff Publications 2021
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Behavioral adaptations imply a direct link between ecologial specialization and reproductive isolation in a sympatrically diverging ground beetle.
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RBINS Staff Publications 2016