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Inproceedings Reference DNA barcoding and diversity of groundwater oligochaetes in Benin (West Africa)
Located in Library / RBINS Staff Publications 2017
Inproceedings Reference Dna barcoding and ecological surveys of the ants of southern Atlantic forest: looking at the tip of the iceberg in a biodiversity hotspot
Located in Library / RBINS Staff Publications 2017
Inproceedings Reference DNA barcoding and ecological survey of the ants of Iguazu National Park: looking at the tip of the iceberg in a biodiversity hotspot
Understanding patterns of species diversity can only be achieved by long-term research and the integration of taxonomical, ecological and behavioral data. Here we studied the diversity and ecology of ants of Iguazu National Park (INP), Argentina, using six sampling techniques. DNA barcodes were used to uncover cryptic diversity, test species/morphospecies delimitation accuracy, and link unidentified male and queen specimens with their worker caste. Results: INP houses 195 described ant species and an additional 49 morphospecies. Leaf litter sampling and pitfall traps were the most efficient sampling methods, while surface baiting revealed the prevalence of large predatory species at different times of the day. Comparing baiting to other sampling methods provided information on species co-existence and the presence of possible dominance hierarchies among ant species. We obtained the DNA barcodes of 312 specimens from 124 species (51% of the ants of INP). Our analyses evidenced a clear barcode gap in all species but two, with an average distance to the nearest neighbor of 15.75%, almost eight times larger than the mean distance to the furthest conspecific (2.07%). Eighty-three percent of the sequence clusters obtained with different clustering algorithms (ABGD, RESL, TCS) matched the reference species or morphospecies, while 10% highlighted possible cryptic diversity. In terms of efficacy, this barcode library allowed a correct identification in more than 94% of the species/morphospecies, and to assign a species name to 69% of the unidentified males and queens. Significance: This study evidences that DNA barcodes are a valuable tool for identifying the ants of the Atlantic Forest, a global diversity hotspot. Furthermore, our project provides a framework for understanding the ecology and the taxonomic diversity of the ants of this region, including the identification of currently undescribed reproductive castes and the discovery of possible cryptic species.
Located in Library / RBINS Staff Publications 2017
Article Reference DNA barcoding and evolutionary relationships in Accipiter Brisson, 1760 (Aves, Falconiformes: Accipitridae) with a focus on African and Eurasian representatives
Located in Library / RBINS Staff Publications
Article Reference DNA barcoding and male genital morphology reveal five new cryptic species in the West palearctic bee Seladonia smaragdula (Vachal, 1895) (Hypmenoptera: Apoidea: Halictidae)
Located in Library / RBINS Staff Publications
Article Reference DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae)
Located in Library / RBINS Staff Publications
Article Reference DNA barcoding echinoderms from the East Coast of South Africa. The challenge to maintain DNA data connected with taxonomy
Echinoderms are marine water invertebrates that are represented by more than 7000 extant species, grouped in five classes and showing diverse morphologies (starfish, sea lilies, feather stars, sea urchins, sea cucumbers, brittle and basket stars). In an effort to further study their diversity, DNA barcodes (DNA fragments of the 5’ end of the cytochrome c oxidase subunit I gene, COI) have been used to complement morphological examination in identifying evolutionary lineages. Although divergent clusters of COI sequences were reported to generally match morphological species delineations, they also revealed some discrepancies, suggesting overlooked species, ecophenotypic variation or multiple COI lineages within one species. Here, we sequenced COI fragments of 312 shallow-water echinoderms of the East Coast of South Africa (KwaZulu-Natal Province) and compared morphological identifications with species delimitations obtained with four methods that are exclusively based on COI sequences. We identified a total of 103 morphospecies including 18 that did not exactly match described species. We also report 46 COI sequences that showed large divergences (>5% p-distances) with those available to date and publish the first COI sequences for 30 species. Our analyses also identified discordances between morphological identifications and COI-based species delimitations for a considerable proportion of the morphospecies studied here (49/103). For most of them, further investigation is necessary to keep a sound connection between taxonomy and the growing importance of DNA-based research.
Located in Library / RBINS Staff Publications 2022
Inproceedings Reference DNA barcoding echinoderms of the East Coast of South Africa
Located in Library / RBINS Staff Publications 2017
Article Reference DNA barcoding fishes from the Congo and the Lower Guinean provinces: Assembling a reference library for poorly inventoried fauna
Abstract The Congolese and Lower Guinean ichthyological provinces are understudied hotspots of the global fish diversity. Here, we barcoded 741 specimens from the Lower and Middle Congo River and from three major drainage basins of the Lower Guinean ichthyological province, Kouilou-Niari, Nyanga and Ogowe. We identified 194 morphospecies belonging to 82 genera and 25 families. Most morphospecies (92.8%) corresponded to distinct clusters of DNA barcodes. Of the four morphospecies present in both neighbouring ichthyological provinces, only one showed DNA barcode divergence <2.5%. A small fraction of the fishes barcoded here (12.9% of the morphospecies and 16.1% of the barcode clusters representing putative species) were also barcoded in a previous large-scale DNA analysis of freshwater fishes of the Lower Congo published in 2011 (191 specimens, 102 morphospecies). We compared species assignments before and after taxonomic updates and across studies performed by independent research teams and observed that most cases of inconsistent species assignments were due to unknown diversity (undescribed species and unknown intraspecific variation). Our results report more than 17 putative new species and show that DNA barcode data provide a measure of genetic variability that facilitates the inventory of underexplored ichthyofaunae. However, taxonomic scrutiny, associated with revisions and new species descriptions, is indispensable to delimit species and build a coherent reference library.
Located in Library / RBINS Staff Publications 2019
Inproceedings Reference DNA barcoding halictine bee species from Europe and Africa
Located in Library / RBINS Staff Publications 2017