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Article Reference DNA barcoding fishes from the Congo and the Lower Guinean provinces: Assembling a reference library for poorly inventoried fauna
Abstract The Congolese and Lower Guinean ichthyological provinces are understudied hotspots of the global fish diversity. Here, we barcoded 741 specimens from the Lower and Middle Congo River and from three major drainage basins of the Lower Guinean ichthyological province, Kouilou-Niari, Nyanga and Ogowe. We identified 194 morphospecies belonging to 82 genera and 25 families. Most morphospecies (92.8%) corresponded to distinct clusters of DNA barcodes. Of the four morphospecies present in both neighbouring ichthyological provinces, only one showed DNA barcode divergence <2.5%. A small fraction of the fishes barcoded here (12.9% of the morphospecies and 16.1% of the barcode clusters representing putative species) were also barcoded in a previous large-scale DNA analysis of freshwater fishes of the Lower Congo published in 2011 (191 specimens, 102 morphospecies). We compared species assignments before and after taxonomic updates and across studies performed by independent research teams and observed that most cases of inconsistent species assignments were due to unknown diversity (undescribed species and unknown intraspecific variation). Our results report more than 17 putative new species and show that DNA barcode data provide a measure of genetic variability that facilitates the inventory of underexplored ichthyofaunae. However, taxonomic scrutiny, associated with revisions and new species descriptions, is indispensable to delimit species and build a coherent reference library.
Located in Library / RBINS Staff Publications 2019
Article Reference DNA barcoding for forensics: experiences from three years of BopCo
Located in Library / RBINS Staff Publications 2019
Inproceedings Reference DNA barcoding identifies an introduced hover fly species (Diptera: Syrphidae: Syrphinae) in the Afrotropics
Located in Library / RBINS Staff Publications
Inproceedings Reference DNA barcoding in European and African Accipiter (Accipitridae: Falconiformes)
Located in Library / RBINS Staff Publications
Article Reference DNA barcoding Madagascar’s amphibian fauna
Located in Library / RBINS Staff Publications
Inproceedings Reference DNA barcoding of ants from the Galapagos Archipelago: searching endemic and introduced species
Located in Library / RBINS Staff Publications 2017
Inproceedings Reference DNA barcoding of fire and thief ants (genus Solenopsis) of the Ecuadorian Andes as a tool for biodiversity research
DNA BARCODING OF FIRE ANTS AND THIEF ANTS (GENUS SOLENOPSIS) OF THE ECUADORIAN ANDES AS A TOOL FOR BIODIVERSITY RESEARCH SONET, G. (1), Nagy, Z. T. (1), Jacquemin, J. (2), Wauters, N. (2), Delsinne, T. (2), Leponce, M. (2) (1) Joint Experimental Molecular Unit, Royal Belgian Institute of Natural Sciences, Brussels & Royal Museum for Central Africa, Tervuren, Belgium (2) Royal Belgian Institute of Natural Sciences & Université Libre de Bruxelles, Belgium Lightning Talk, Barcoding Insects 2, Napier 208, Friday, 15:00 to 15:07 Poster Location: B25 Members of the genus Solenopsis are among the most abundant ants in tropical rainforests. They are represented by more than 200 described species worldwide and some are dreadful invasive species. The identification to the species level is hampered by a dearth of diagnostic morphological characters and represents a serious limitative step in biodiversity inventories and in the study of invasive species. We set up and validated a DNA barcoding procedure to identify ants of the genus Solenopsis collected in the Podocarpus National Park of the Ecuadorian Andes. Complete specimens were used for DNA extraction and subsequently preserved as vouchers to allow further morphological analysis. More than 14 new molecular operational taxonomic units were identified by the standard DNA barcode fragment. In some cases specimens from a single morpho-species occurring at different altitudes could be distinguished. This study resulted in an appropriate laboratory protocol and a reference library useful to identify ants of the genus Solenopsis in the Ecuadorian Andes.
Located in Library / RBINS Staff Publications
Inproceedings Reference DNA barcoding of reptiles: practical aspects
Located in Library / RBINS Staff Publications
Article Reference DNA barcoding reveals new insights into the diversity of Antarctic species of Orchomene sensu lato (Crustacea: Amphipoda: Lysianassoidea)
Located in Library / RBINS Staff Publications
Article Reference DNA barcoding the Lake Edward basin: high taxonomic coverage of a tropical freshwater ichthyofauna
Located in Library / RBINS Staff Publications 2022