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Article Reference chemical/x-molconn-Z Exploring the use of Cytochrome Oxidase c Subunit 1 (COI) for DNA barcoding of free-living marine nematodes
Background: The identification of free-living marine nematodes is difficult because of the paucity of easily scorable diagnostic morphological characters. Consequently, molecular identification tools could solve this problem. Unfortunately, hitherto most of these tools relied on 18S rDNA and 28S rDNA sequences, which often lack sufficient resolution at the species level. In contrast, only a few mitochondrial COI data are available for free-living marine nematodes. Therefore, we investigate the amplification and sequencing success of two partitions of the COI gene, the M1-M6 barcoding region and the I3-M11 partition. Methodology: Both partitions were analysed in 41 nematode species from a wide phylogenetic range. The taxon specific primers for the I3-M11 partition outperformed the universal M1-M6 primers in terms of amplification success (87.8\% vs. 65.8\%, respectively) and produced a higher number of bidirectional COI sequences (65.8\% vs 39.0\%, respectively). A threshold value of 5\% K2P genetic divergence marked a clear DNA barcoding gap separating intra-and interspecific distances: 99.3\% of all interspecific comparisons were 〉0.05, while 99.5\% of all intraspecific comparisons were 〈0.05 K2P distance. Conclusion: The I3-M11 partition reliably identifies a wide range of marine nematodes, and our data show the need for a strict scrutiny of the obtained sequences, since contamination, nuclear pseudogenes and endosymbionts may confuse nematode species identification by COI sequences.
Located in Library / No RBINS Staff publications
Article Reference Benthic effects of offshore renewables: identification of knowledge gaps and urgently needed research
Located in Library / RBINS Staff Publications 2019
Article Reference A new inioid (Cetacea, Odontoceti, Delphinida) from the Miocene of Peru and the origin of modern dolphin and porpoise families
Located in Library / RBINS Staff Publications 2017
Article Reference Dispersal models alert on the risk of non-native species introduction by Ballast water in protected areas from the Western Antarctic Peninsula
Located in Library / RBINS Staff Publications 2022
Article Reference Extension of the leafhopper genus Multinervis (Hemiptera, Cicadellidae, Megophthalminae, Agalliini) from Northern to Central Vietnam, with the description of one new species
Located in Library / RBINS Staff Publications 2025
Article Reference First record of the cicadas genus Orientopsaltria Kato, 1944 (Hemiptera: Cicadidae) from Vietnam, with description of one new species
Located in Library / RBINS Staff Publications 2019
Article Reference New marine Thinophilus species (Diptera: Dolichopodidae: Hydrophorinae) from the Thai Andaman Sea coast and new records from peninsular Thailand
Located in Library / No RBINS Staff publications
Article Reference New Late Cretaceous dinosaur findings from northwestern Transylvania (Romania).
Located in Library / RBINS Staff Publications
Article Reference A new species of Velociraptor (Dinosauria: dromaeosauridae) from the Upper Cretaceous of Northern China
Located in Library / RBINS Staff Publications
Article Reference New hadrosaurid dinosaurs from the uppermost Cretaceous of northeastern China
Located in Library / RBINS Staff Publications