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EVOSHEEP: the makeup of sheep breeds in the ancient Near East
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The EVOSHEEP project combines archaeozoology, geometric morphometrics and genetics to study archaeological sheep assemblages dating from the sixth to the first millennia BC in eastern Africa, the Levant, the Anatolian South Caucasus, the Iranian Plateau and Mesopotamia. The project aims to understand changes in the physical appearance and phenotypic characteristics of sheep and how these related to the appearance of new breeds and the demand for secondary products to supply the textile industry.
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RBINS Staff Publications 2021
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Exceptional cryptic diversity and multiple origins of parthenogenesis in a freshwater ostracod
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RBINS Staff Publications
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Exchange processes and nitrogen cycling on the shelf and continental slope of the Black Sea basin.
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A 3D coupled biogeochemical-hydrodynamical model has been applied to the Black Sea to simulate nitrogen cycling and to estimate the exchange of biogeochemical components at the shelf break and between the continental slope and the deep sea. It was found that biological processes on the northwestern shelf are in approximate balance. Primary production is fueled by river discharge, nitrate input from the open sea at the shelf break, and in situ remineralization. The input of nitrate from the open sea is roughly equivalent to the river nitrate discharge but is half the nitrate export from the shelf toward the open sea. Also, the Black Sea shelf acts throughout the year as a nitrate source for the open sea. The amount of shelf production not remineralized in the euphotic layer is 22.2% and is exported to lower layers (20%) or offshore (2.2%). We estimate that the export of carbon from the shelf to the interior of the basin represents 2.5% of the new production of the open sea. The upper slope adjoining the northwestern shelf is the site of downwelling events responsible for the downward transport to the intermediate layer of the continental slope of biogeochemical components exported from the shelf in the upper layer. The shelf has been found to be an efficient trap for the refractory material discharged by the Danube.
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Expanding the swimmer’s itch pool of the Benelux: a first record of the neurotropic Trichobilharzia regenti and potential link to human infection
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RBINS Staff Publications 2024
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Experimental exposure to cadmium affects metallothionein-like protein levels but not survival and growth in wolf spiders from polluted and reference populations
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Both local adaptation and acclimation in tolerance mechanisms may allow populations to persist under metal pollution. However, both mechanisms are presumed to incur (energetic) costs and to trade-off with other life-history traits. To test this hypothesis, we exposed Pardosa saltans (Lycosidae) spiderlings originating from metal-polluted and unpolluted sites to a controlled cadmium (Cd) treatment, and compared contents of metal-binding metallothionein-like proteins (MTLPs), internal metal concentrations, and individual survival and growth rates with a reference treatment. While increased MTLP concentrations in offspring originating from both polluted and unpolluted populations upon exposure indicates a plastic tolerance mechanism, survival and growth rates remain largely unaffected, independent of the population of origin. However, MTLP and Cd concentrations were not significantly correlated. We suggest that MTLP production may be an important mechanism enabling P. saltans populations to persist in ecosystems polluted with heavy metals above a certain level. (C) 2010 Elsevier Ltd. All rights reserved.
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RBINS Staff Publications
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Experimental infection of Carrion crows (Corvus corone) with two European West Nile virus (WNV) strains.
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RBINS Staff Publications
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Experimental metamorphosis of Halisarca dujardini larvae (Demospongiae, Halisarcida): Evidence of flagellated cell totipotentiality
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The potency of flagellated cells of Halisarca dujardini (Halisarcida, Demospongiae) larvae from the White Sea (Arctic) was investigated experimentally during metamorphosis. Two types of experiments were conducted. First, larvae were maintained in Ca2+ free seawater (CFSW) until the internal cells were released outside through the opening of the posterior pole. These larvae that only composed of flagellated cells (epithelial larvae) were then returned to sea water (SW) to observe their metamorphosis. The posterior aperture closed before they settled on a substratum and started a metamorphosis similar to intact larvae. Secondly, epithelial larvae were, first, further treated in CFSW and then mechanically dissociated. Separated cells or groups of cells were returned to SW, where they constituted large friable conglomerates. After 12-17 h in SW, flagellated cells showed the first steps of dedifferentiation, and regional differentiation was noticeable within conglomerates after approximately 24-36 h. External cells differentiated into pinacocytes while internal cells kept their flagella and became united in a layer. Within 48-72 h, internal cells of the conglomerates formed spherical or ovoid clusters with an internal cavity bearing flagella. These clusters further fused together in a rhagon containing one or two large choanocyte chambers. The sequence of cellular processes in epithelial larvae and in flagellated cell conglomerates was similar. Previous observations indicating the totipotentiality of larval flagellated cells during normal metamorphosis of H. dujardini are thus confirmed. © 2007 Wiley-Liss, Inc.
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RBINS Staff Publications
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Experiments on tsunami induced boulder transport – A review
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RBINS Staff Publications 2021
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Explaining Uncertainty Avoidance in Meciaprojects: Resource Constraints, Strategic Behaviour, or Institutions?
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RBINS Staff Publications 2021
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Exploring hidden parasite diversity with environmental DNA metabarcoding during a ParasiteBlitz across a coastal habitat gradient
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Environmental DNA (eDNA) metabarcoding has lagged in parasite biodiversity assessments. We implemented this method to examine parasite diversity in sediment and water from 4 physically connected aquatic habitats in coastal South Carolina, USA, as part of a ParasiteBlitz in April 2023. Sediment was collected using a syringe corer, and water was sampled using active filtration and passive collection. Five amplicon libraries, using primers targeting portions of the mitochondrial COI of platyhelminths and 18S ribosomal RNA genes of nematodes, myxozoans, microsporidians, and protists, successfully yielded parasite sequences. Out of >5.8 million sequences, we identified >1,000 parasite amplicon sequence variants (ASVs) corresponding to ~600 parasite operational taxonomic units, from 6 parasite groups. Most diversity was observed among the microsporidians, whose assay demonstrated the highest fidelity. Actively-filtered water samples captured ASVs of all 6 groups, whereas sediment captured only 4, despite yielding 3× as many ASVs. Low DNA yields from passive water samples resulted in fewer, but some unique, ASVs representing 3 parasite groups. The most efficient sampling method varied with respect to parasite group across habitats, and the parasite communities from each habitat were distinct regardless of sampling method. We detected ASVs of 9 named species, 4 of which may represent introductions to the US. The abundance of our results demonstrates the effectiveness and efficiency of eDNA metabarcoding for assessing parasite diversity during short, intensive surveys, and highlights the critical need for more comprehensive sequence databases and the development of primers for those parasite taxa that elude detection using eDNA methods.
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RBINS Staff Publications 2025
