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Community ecology of Tanzanian bats and their viruses
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RBINS Staff Publications 2023
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Comparing Gravettian and Epigravettian canids from Europe with Late Pleistocene canids from Yakutia
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RBINS Staff Publications 2016
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Comparing the results of four widely used automated bat identification software programs in the North Sea region
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Recently a few papers were published addressing the prudency needed when using automated identification software programs to analyse recordings of bat echolocation sounds. We want to contribute to that discussion by analysing a reference dataset of bat recordings with four widely used and commercially available software programs (BatIdent, BatExplorer, Kaleidoscope and Sonochiro). The reference data were all recorded in Western-Europe with a batcorder. For most of the recordings there was a visual confirmation of the recorded species. In a few other cases certainty was obtained because the specimens were captured and released or because the recordings were made in certain areas which were outside of the range of other species (e.g. at high altitude to separate Eptesicus serotinus and Eptesicus nilssonii). After running the different programs on the reference data, we compared the outputted results. Overall, identification of the recordings to species level in this test was best with Batident (81% correct identifications), followed by Kaleidoscope (71%), Sonochiro (63%) and BatExplorer (53%). We can conclude that each of the tested programs has its own strengths and weaknesses, but none of them should be used unsupervised. Outputted results need to be checked by a trained expert. In this way, our test affirms the conclusions of previous tests in Northern Europe and the USA.
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RBINS Staff Publications 2017
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Comparing the transposon landscapes of a putative ancient asexual and a sexual non-marine ostracod (Crustacea, Arthropoda)
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Ostracods are microscopic, bi-valved crustaceans with the best fossil record of all living arthropods. Their fossil record, starting 400 million years ago, together with a high prevalence of parthenogenetic reproduction and putative ancient asexuality, make non-marine ostracods fascinating evolutionary model organisms. In the absence of high quality ostracod reference genomes, here we compare transposon landscapes between two Illumina genome assemblies from the putative ancient asexual Darwinula stevensoni and the fully sexual ostracod Notodromas monacha. Both assemblies have around 60,000 contigs, sizes of 360-380 Mb, more than 100X coverage and BUSCO scores of 93 and 94%, respectively. Because homology-based programs are not sensitive enough to detect families of transposable elements (TEs) in species missing from Repbase or Dfam, we used three different pipelines for de novo analyses: REPET, RepeatMasker2 (RM2) and EarlGrey (RM2-based, with automated curation). TE diversity between the two genomes differs substantially regardless which pipeline was used. The Illumina assembly of N. monacha is dominated by LTR retrotransposons (6.5%) with some DNA transposons (3.7%), whereas DNA (15.5%), LINE-like (5.9%) and rolling circle Helitron elements (1.5%) were most abundant in the assembly of D. stevensoni. Our results on the dominance of DNA (Tc/mar, hAT) and LINE-like (CR1, RTE) TEs in D. stevensoni parallel earlier findings from a partial genomic library, and differ from those of other asexuals. TE copies with a low number of nucleotide substitutions are only observed with REPET (“L” shape landscape) in both genomes. Although the presented results may underestimate TE abundance, they indicate pronounced differences of the transposon landscapes and diversity between these two ostracod species. Analysis of related species should determine whether the differences are correlated with the reproductive mode or are lineage specific.
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RBINS Staff Publications 2024
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Comparing the transposon landscapes of a putative ancient asexual and a sexual non-marine ostracod (Crustacea, Arthropoda)
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Ostracods are microscopic, bi-valved crustaceans with the best fossil record of all living arthropods. Their fossil record, starting 400 million years ago, together with a high prevalence of parthenogenetic reproduction and putative ancient asexuality, make non-marine ostracods fascinating evolutionary model organisms. In the absence of high quality ostracod reference genomes, we here compare transposon landscapes between two Illumina genome assemblies from the putative ancient asexual Darwinula stevensoni and the fully sexual ostracod Notodromas monacha. Both assemblies have around 60,000 contigs, sizes of 360-380 Mb, more than 100X coverage and BUSCO scores of 93 and 94%, respectively. Because homology-based programs are not sensitive enough to detect families of transposable elements (TEs) in species missing from Repbase or Dfam, we used three different pipelines for de novo analyses: REPET, RepeatMasker2 (RM2) and EarlGrey (RM2-based, with automated curation). TE diversity between the two genomes differs substantially regardless which pipeline was used. The Illumina assembly of N. monacha is dominated by LTR retrotransposons (6.5%) with some DNA transposons (3.7%), whereas DNA (15.5%), LINE-like (5.9%) and rolling circle Helitron elements (1.5%) were most abundant in the assembly of D. stevensoni. Our results on the dominance of DNA (Tc/mar, hAT) and LINE-like (CR1, RTE) TEs in D. stevensoni parallel earlier findings from a partial genomic library, and differ from those of other asexuals. TE copies with a low number of nucleotide substitutions are only observed with REPET (“L” shape landscape) in both genomes. Although the presented results may underestimate TE abundance, they indicate pronounced differences of the transposon landscapes and diversity between these two ostracod species. Analysis of related species should determine whether the differences are correlated with the reproductive mode or are lineage specific. We are currently curating TEs in an Oxford Nanopore draft assembly of D. stevensoni to further confirm our initial results.
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RBINS Staff Publications 2024
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Comparing the transposon landscapes of a putative ancient asexual and a sexual non-marine ostracod (Crustacea, Arthropoda)
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Ostracods are microscopic, bi-valved crustaceans with the best fossil record of all living arthropods. Their fossil record, starting 400 million years ago, together with a high prevalence of parthenogenetic reproduction and putative ancient asexuality, make non-marine ostracods fascinating evolutionary model organisms. In the absence of high quality ostracod reference genomes, we here compare transposon landscapes between two Illumina genome assemblies from the putative ancient asexual Darwinula stevensoni and the fully sexual ostracod Notodromas monacha. Both assemblies have around 60,000 contigs, sizes of 360-380 Mb, more than 100X coverage and BUSCO scores of 93 and 94%, respectively. Because homology-based programs are not sensitive enough to detect families of transposable elements (TEs) in species missing from Repbase or Dfam, we used three different pipelines for de novo analyses: REPET, RepeatMasker2 (RM2) and EarlGrey (RM2-based, with automated curation). TE diversity between the two genomes differs substantially regardless which pipeline was used. The Illumina assembly of N. monacha is dominated by LTR retrotransposons (6.5%) with some DNA transposons (3.7%), whereas DNA (15.5%), LINE-like (5.9%) and rolling circle Helitron elements (1.5%) were most abundant in the assembly of D. stevensoni. Our results on the dominance of DNA (Tc/mar, hAT) and LINE-like (CR1, RTE) TEs in D. stevensoni parallel earlier findings from a partial genomic library, and differ from those of other asexuals. TE copies with a low number of nucleotide substitutions are only observed with REPET (“L” shape landscape) in both genomes. Although the presented results may underestimate TE abundance, they indicate pronounced differences of the transposon landscapes and diversity between these two ostracod species. Analysis of related species should determine whether the differences are correlated with the reproductive mode or are lineage specific. We are currently curating TEs in an Oxford Nanopore draft assembly of D. stevensoni to further confirm our initial results.
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RBINS Staff Publications 2024
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COMPARISON OF ONSHORE AND OFFSHORE BIRD MIGRATION BY DIFFERENT RADAR SYSTEMS NEAR THE BELGIAN COAST
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Comparison of spatial genetic structure and its drivers in Arctic and Antarctic fishes
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Marine populations are genetically structured through historical processes, environmental or physical barriers and life history characteristics. Divergent patterns of demographic history, even among closely-related species sharing climatic changes, raise questions about the influence of species-specific traits on population structure. The Southern Ocean features comparatively high biodiversity, which has been attributed to frequent local extinction-recolonization cycles that have driven benthic, Antarctic organisms into temporary refugia. In contrast, organisms in the Arctic were able to shift latitude in response to changing Pleistocene climate. We therefore hypothesize that Arctic populations were historically less constrained in their distribution than Antarctic fish populations and hence show lower levels of genetic structure. For assessing the role of lifestyle in influencing demographic history in the Southern Ocean closely related notothenioid fish with benthic (Trematomus bernacchii, T. hansoni) and semi-pelagic or even cryopelagic (T. newnesi) lifestyles were genetically analysed. In the Arctic, polar cod (Boreogadus saida), which is often found in association with sea ice, but also throughout the water column to the bottom, can be regarded as semi- or cryopelagic too. The Antarctic species were analysed by six microsatellite and one mitochondrial marker before (Van de Putte et al., 2012) and we extend these analyses with data from nine microsatellite markers in polar cod. Antarctic species showed significant genetic population structure between High-Antarctic and Peninsular regions and much lower differentiation in pelagic than benthic species. It suggests that the observed patterns are indeed related to ecological traits of Antarctic fish. In the Arctic, we hypothesize genetic structuring inside fjords in Svalbard relative to shelf specimens, which we expect to show low or absent structure as in Antarctic species with a similar lifestyle. Identifying common driving factors for population structure is important in order to enable forecasting, particularly in light of dramatically increasing rates of environmental change. Comparing population genetic patterns and exploring underlying causes from both poles may thus help to shed light on how fish populations survived in the past and may persist in the future. Reference - Van de Putte A., Janko K., Kasparova E., Maes G.E., Rock, J., Koubbi P., Volckaert F.A.M., Choleva L., Fraser K.P.P., Smykla J., Van Houdt J.K.J., Marshall C. 2012 Comparative phylogegraphy of three trematomid fishes reveals contrasting genetic structure patterns in benthic and pelagic species. Marine Genomics 8:23-34.
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RBINS Staff Publications 2018
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Competent Authority: Are They Ready to Evaluate Applications?
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RBINS Staff Publications
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Competition among Neo-Guinean arboreal termites with different dispersal and warfare strategies.
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No RBINS Staff publications