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Article Reference Ancient RNA from Late Pleistocene permafrost and historical canids shows tissue-specific transcriptome survival
While sequencing ancient DNA (aDNA) from archaeological material is now commonplace, very few attempts to sequence ancient transcriptomes have been made, even from typically stable deposition environments such as permafrost. This is presumably due to assumptions that RNA completely degrades relatively quickly, particularly when dealing with autolytic, nuclease-rich mammalian tissues. However, given the recent successes in sequencing ancient RNA (aRNA) from various sources including plants and animals, we suspect that these assumptions may be incorrect or exaggerated. To challenge the underlying dogma, we generated shotgun RNA data from sources that might normally be dismissed for such study. Here, we present aRNA data generated from two historical wolf skins, and permafrost-preserved liver tissue of a 14,300-year-old Pleistocene canid. Not only is the latter the oldest RNA ever to be sequenced, but it also shows evidence of biologically relevant tissue specificity and close similarity to equivalent data derived from modern-day control tissue. Other hallmarks of RNA sequencing (RNA-seq) data such as exon-exon junction presence and high endogenous ribosomal RNA (rRNA) content confirms our data’s authenticity. By performing independent technical library replicates using two high-throughput sequencing platforms, we show not only that aRNA can survive for extended periods in mammalian tissues but also that it has potential for tissue identification. aRNA also has possible further potential, such as identifying in vivo genome activity and adaptation, when sequenced using this technology.
Located in Library / RBINS Staff Publications 2019
Inbook Reference text/h323 Des pots dans les minières. La céramique Michelsberg de Spiennes
Located in Library / RBINS Staff Publications 2016
Inbook Reference Datations radiocarbones du Néolithique ancien entre Bassin parisien et Bassin rhénan. Prolégomènes théoriques, applications pratiques et après…
Located in Library / RBINS Staff Publications 2016
Article Reference Radiocarbon chronology for the Early Gravettian of northern Europe: new AMS determinations for Maisières-Canal, Belgium
Located in Library / RBINS Staff Publications 2016
Article Reference Quévy/Givry et Havay : fouille d’un fossé circulaire
Located in Library / RBINS Staff Publications 2016
Article Reference Datations radiocarbones d’ossements humains du site minier néolithique de Spiennes (Mons, Hainaut). Première approche
Located in Library / RBINS Staff Publications 2016
Article Reference Inter-site analysis of armatures from five Linearbandkeramik settlements in the Hesbaye region
Located in Library / RBINS Staff Publications 2016
Article Reference Datation d’un fragment de métatarsien humain découvert anciennement dans les minières de «Petit-Spiennes»
Located in Library / RBINS Staff Publications 2016
Article Reference Les ossements humains des minières néolithiques de silex de Spiennes : le début d’une enquête
Located in Library / RBINS Staff Publications 2016
Article Reference Identification des phases minérales par DRX et EDS dans les processus d'altération des pyrites et des marcassites : applications aux collections de Préhistoire, de Paléontologie et de Minéralogie de l’IRSNB aux briquets
Located in Library / RBINS Staff Publications 2016