Search publications of the members of the Royal Belgian institute of natural Sciences
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Revision of some species of the genus Paralongidorus sensu Siddiqi et al. (1993), with a discussion on the relationships within the family Longidoridae (Nematoda: Dorylaimida)
- A study of type material of several longidorid species revealed misinterpretations of the amphid structure with respect to shape of the amphidial fovea and amphidial aperture. A standardised terminology of the amphid structure within the Longidoridae is proposed. Former and recent hypotheses on phylogenetic relationships within the family based, respectively, on morphological and on molecular data are discussed, as well as possible implications for the systematics of the family Longidoridae. As a result of this study the following new combinations are proposed: Longidorus boshi n. comb. (syn. Paralongidorus boshi); Longidorus monegrensis n. comb. (syn. P. monegrensis); Longidorus spiralis n. comb. (syn. P. spiralis) and Longidorus cedari n. comb. (syn. Longidoroides cedari). Additional information is provided for Longidoroides bikanerensis, Paralongidorus buchae and P. zenobiae. © 2007 Brill Academic Publishers.
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Ant biodiversity conservation in Belgian calcareous grasslands: Active management is vital
- A list of ant species collected in eight calcareous grasslands in the Viroin valley (Viroinval, Belgium) is presented. Thirty species were identified, including Temnothorax albipennis, for the first time recorded in Belgium. Ant community composition and chorology of some ant species are discussed. Recommendations on how to use ant community composition and nest densities of several ant species to evaluate management in calcareous grasslands are given. It appears that in locations with encroachment of tall grasses (especially Brachypodium pinnatum) and spontaneous afforestation, due to a complete lack of or to inadequate management, most of the often rare xerophilic ant species are replaced by mesophilic, rather common species.
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Spatiotemporal analysis of population genetic structure in Geomonhystera disjuncta (Nematoda, Monhysteridae) reveals high levels of molecular diversity
- Species identification in the phylum Nematoda is complicated due to the paucity of easily obtainable diagnostic morphological features. Furthermore, the cosmopolitan distribution of several species despite low dispersal abilities makes cryptic diversity potentially substantial within this phylum. We conducted a population genetic survey in the marine nematode Geomonhystera disjuncta in Belgium and The Netherlands in two seasons. The mitochondrial cytochrome oxidase c subunit 1 (COI) gene was screened with the single-strand conformation polymorphism method in 759 individuals. The 43 haplotypes were grouped into five lineages, with low divergences within (3\%) and high divergences between lineages (14\%). Analysis of the nuclear ITS region yielded concordant tree topologies, indicating the presence of five cryptic taxa within G. disjuncta. Analysis of Molecular Variance (AMOVA) illustrated a significant structuring in all lineages and temporal fluctuations in haplotype frequencies within and between locations. Metapopulation dynamics and/or priority effects best explained this structuring. Finally, our data indicate that the COI gene may be useful for DNA barcoding purposes. © 2007 Springer-Verlag.
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Inter- And intraspecific genetic and morphological variation in a sibling pair of carabid species
- Background: Pogonus littoralis and Pogonus chalceus are very close related species with quite different ecological preferences within salt marshes. We study the evolutionary processes in and between these presumably young species. Therefore, we compare the variation in ecologically relevant characters and the genetic variation within one of the species (intraspecific differentiation) with the variation of the two types of characters between the two species (interspecific variation). Data are compared between two independent sets of populations, one set at a small geographical scale (the ecologically diverse Guérande area in France) and the other set at a Atlantic-Mediterranean scale. Results: Body and relative wing size and IDHI allozyme data show that the intraspecific variation in P. chalceus is high and in the same range as the interspecific variation (P. chalceus versus P. littoralis). Based on neutral markers (other allozymes and mitochondrial DNA) on the other hand, the intraspecific variation in P. chalceus is much lower in comparison to the interspecific variation. Conclusion: The different ecotypes in the highly polytypic species P. chalceus are as highly differentiated in ecological characters as true species, but are not recognised as such by screening neutral DNA polymorphisms. This can be interpreted as a case of ongoing speciation driven by natural selection adapting each ecotype to its respective ecological niche. The same ecological process can be recognised in the differentiation between the two sister species, where en plus reproductive isolation between the two gene pools occurred, allowing independent drift and mutation accumulation in neutral genetic characters.
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Experimental metamorphosis of Halisarca dujardini larvae (Demospongiae, Halisarcida): Evidence of flagellated cell totipotentiality
- The potency of flagellated cells of Halisarca dujardini (Halisarcida, Demospongiae) larvae from the White Sea (Arctic) was investigated experimentally during metamorphosis. Two types of experiments were conducted. First, larvae were maintained in Ca2+ free seawater (CFSW) until the internal cells were released outside through the opening of the posterior pole. These larvae that only composed of flagellated cells (epithelial larvae) were then returned to sea water (SW) to observe their metamorphosis. The posterior aperture closed before they settled on a substratum and started a metamorphosis similar to intact larvae. Secondly, epithelial larvae were, first, further treated in CFSW and then mechanically dissociated. Separated cells or groups of cells were returned to SW, where they constituted large friable conglomerates. After 12-17 h in SW, flagellated cells showed the first steps of dedifferentiation, and regional differentiation was noticeable within conglomerates after approximately 24-36 h. External cells differentiated into pinacocytes while internal cells kept their flagella and became united in a layer. Within 48-72 h, internal cells of the conglomerates formed spherical or ovoid clusters with an internal cavity bearing flagella. These clusters further fused together in a rhagon containing one or two large choanocyte chambers. The sequence of cellular processes in epithelial larvae and in flagellated cell conglomerates was similar. Previous observations indicating the totipotentiality of larval flagellated cells during normal metamorphosis of H. dujardini are thus confirmed. © 2007 Wiley-Liss, Inc.
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Esperiopsis koltuni sp. nov. (Demospongiae: Poecilosclerida: Esperiopsidae), a carnivorous sponge from deep water of the Sea of Okhotsk (North Pacific)
- Esperiopsis koltuni, a new species of the sponge family Esperiopsidae, is described from deep water of the Sea of Okhotsk. The new species has a unique growth form: there is a basal plate with radiating cylindrical branches, whose oval flat distal parts bear filament-like outgrowths. Megascleres are arranged differently in the main part of branches and in their distal flat parts. The dermal membrane contains isochelae with protruding alae and abundant sigmancistras. Microscleres are represented by large anchorate spatuliferous isochelae, small isochelae, sigmas and sigmancistras. Esperiopsis koltuni sp. nov. is hermaphroditic, with parenchymella larva. The larval skeleton consists only of sigmas and sigmancistras. Esperiopsis koltuni sp. nov. can be easily distinguished from other Esperiopsis species by dimensions and combination of spicule types. In particular, it is the only species in the genus with three different size categories of styles. The new species lacks the aquiferous system, has a characteristic body plan with symmetrical lateral expansions, an unusual arrangement of microscleres in the dermal membrane and true sigmancistras. Moreover, prey capture by a filament-like outgrowth of the flat distal part was observed. All of these characteristics indicate that Esperiopsis koltuni sp. nov. is a carnivorous sponge.
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An estimate of the suspended particulate matter (SPM) transport in the southern North Sea using SeaWiFS images, in situ measurements and numerical model results
- A study is presented where satellite images (SeaWiFS), in situ measurements (tidal cycle and snapshot) and a 2D hydrodynamic numerical model have been combined to calculate the long term SPM (Suspended Particulate Matter) transport through the Dover Strait and in the southern North Sea. The total amount of SPM supplied to the North Sea through the Dover Strait is estimated to be 31.74×106 t. The satellite images provide synoptic views of SPM concentration distribution but do not take away the uncertainty of SPM transport calculation. This is due to the fact that SPM concentration varies as a function of tide, wind, spring-neap tidal cycles and seasons. The short term variations (tidal, spring-neap tidal cycle) have not been found in the satellite images, however seasonal variations are clearly visible. Furthermore the SPM concentration in the satellite images is generally lower than in the in situ measurements. The representativness of SPM concentration maps derived from satellites for calculating long term transports has therefore been investigated by comparing the SPM concentration variability from the in situ measurements with those of the remote sensing data. The most important constraints of satellite images are related to the fact that satellite data is evidence of clear sky conditions, whereas in situ measurements from a vessel can be carried out also during rougher meteorological conditions and that due to the too low time resolution of the satellite images the SPM concentration peaks are often missed. It is underlined that SPM concentration measurements should be carried out during at least one tidal cycle in high turbidity areas to obtain representative values of SPM concentration. © 2007 Elsevier Ltd. All rights reserved.
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Antrobathynella stammeri (Jakobi, 1954) : The first record of bathynellacea (Crustacea : Syncarida) in Belgium
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Fossil bear bones in the Belgian Upper Paleolithic: The possibility of a proto bear-ceremonialism
- This paper examines several fossil bear bones from Upper Paleolithic deposits recovered from Belgian caves. The bones exhibit traces of ochre. The paper examines the archaeological and archaeozoological data in detail, comparing the finds with other sites containing fossil bear remains and identifying patterns suggesting purposeful staining of bear bones. These patterns are compared with ethnographic examples of bear ceremonialism. On the basis of similarities found in these analyses, the authors argue that a proto bear-ceremonialism existed during the Upper Paleolithic. © 2007 by the Board of Regents of the University of Wisconsin System.
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Spiniferaphonte, a new genus of Laophontidae (Copepoda: Harpacticoida), with notes on the occurrence of processes on the caudal rami
- A new genus and species of Laophontidae, Spiniferaphonte ornata n. gen., n. sp., is described from the coast of Kenya. The new genus is closely related to Laophontina and Wellsiphontina as shown by the following synapomorphies: a denticulate operculum, a sexually dimorphic P4 exopod (reduced chaetotaxy of the ultimate segment in the male), and the absence of sexual dimorphism in the P2 and P3 endopods. The two-segmented exopod of P1 and the presence of a seta on the endopodal part of the male P5 are plesiomorphies indicating that the new genus represents a separate lineage within this group. The proposal of the new genus Spiniferaphonte is supported by the following autapomorphies: three smooth setae on the female P5 exopod and a robust, dorsally bent, and strongly sclerotised caudal seta V. Within the Laophontidae, it is striking that the presence of distinct, thorn-like processes on the caudal rami is limited to interstitial genera. Distinct processes on the proximal segments of the antennule and a proximally thickened caudal seta V also appear to be associated with this interstitiality. These structures may play a role in the movement and the anchoring of the animals in their interstitial habitat.
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Revision of the genus Tapholeon Wells, 1967 (Copepoda, Harpacticoida, Laophontidae)
- To date, only two species are known in the laophontid genus Tapholeon Wells, 1967 (Copepoda, Harpacticoida). In the present contribution, a redescription of the type species T. ornatus Wells, 1967, based on the type material, is provided. Furthermore, two new species are described from the coast of Kenya, T. inconspicuus sp. nov. and T. tenuis sp. nov. Two species, formerly attributed to Asellopsis Brady and Robertson, 1873 (namely A. arenicola Chappuis, 1954 and A. chappuisius Krishnaswamy, 1957), are allocated to Tapholeon based on the absence of sexual dimorphism in the swimming legs P2-P4. The former of the two species is redescribed based on additional material from the Comoros. An updated generic diagnosis and a key to the six species of Tapholeon are included. © 2007 Taylor & Francis.
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Whole-genome shotgun sequencing of mitochondria from ancient hair shafts
- Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.
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The archaebacterial communities in Antarctic bathypelagic sediments
- 16S ribosomal DNA clone library analysis was performed to assess archaeal diversity within three surficial sediment samples obtained from the bathypelagic zone (depth: 2165-3406 m) of the Weddell Sea, Antarctica. The nearly complete 16S rDNA gene (1440 bp) was obtained for 146 clones and 46 phylotypes were defined. The majority of the sequences (99\%) formed three clusters within the Marine Group I Crenarchaeota. The most important cluster, with 78.8\% of the clones, included Candidatus Nitrosopumilus maritimus, a mesophilic archaeon able to oxidize ammonia. The most important subgroup in that cluster was the APA4-0cm subgroup (with 62.3\% of the clones). This subgroup might represent important Crenarchaeota in the functioning of the bathypelagic sedimentary ecosystems of the Weddell Sea because it dominated the clone libraries in all sampling stations, and was found in sediments separated by very large geographic distances. Only one clone grouped within the Euryarchaeota. This euryarchaeal clone could not be affiliated with any of the previously defined clusters and might represent a novel euryarchaeal lineage. © 2007 Elsevier Ltd. All rights reserved.
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Phylogenetic relationships and classification of the Malagasy pseudoxyrhophiine snake genera Geodipsas and Compsophis based on morphological and molecular data
- Based on a specimen found at Montagne d'Ambre in northern Madagascar morphologically agreeing with Compsophis albiventris Mocquard, 1894, we report on the rediscovery of this enigmatic snake genus and species and its molecular phylogenetic relationships. Compsophis albiventris, considered to be the only representative of its genus and unreported since its original description, bears strong morphological similarities to species of Geodipsas Boulenger, 1896. A molecular phylogeny based on DNA sequences of three mitochondrial and nuclear genes (complete cytochrome b, fragments of 16S rRNA and c-mos) in Compsophis albiventris and three Geodipsas species corroborated close relationships between C. albiventris and Geodipsas boulengeri, and showed that the genera Compsophis and Geodipsas together form a monophyletic unit. Despite the general similarities, morphological data and chromatic features support the existence of two species groups, corresponding to Compsophis and Geodipsas. We consequently consider Geodipsas as a subgenus of Compsophis and transfer all species currently in Geodipsas into the genus Compsophis. Copyright © 2007 Magnolia Press.
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Conodont-based graphic correlation of upper Givetian-Frasnian sections of the Eastern anti-atlas (Morocco)
- A high-resolution correlation of ten upper Givetian-Frasnian sections spread over the different facies environments of the Eastern Anti-Atlas (Morocco) is established using graphic correlation. The measured stratigraphic ranges of eighty-five conodont taxa have been assembled into a chronostratigraphic framework. The developed regional composite subdivides the Frasnian into 501 composite standard units (CSU) based on the original thickness of the reference section (Bou Tchrafine). This is a higher resolution than obtained by the classic biozone correlation. Based on the conodont data available for this correlation project, the jamieae and Lower rhenana zones could not be recognised in the sections.
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Non-marine Chersodromia Walker from Southeast Asia and Papua New Guinea (Diptera, Empidoidea, Hybotidae)
- Two small species of Chersodromia Walker are described from lowland rain forest in Papua New Guinea and Thailand: C. flavicaput sp. nov. (Papua New Guinea, Thailand) and C. obscura sp. nov. (Thailand). The species are almost completely white, except for the contrastingly black eyes. Although the eyes touching on the face and wing venation suggest affinities with Nanodromia Grootaert, the extended gena, bristling on the apical half of the hind tibia and structure of the male genitalia indicate inclusion in Chersodromia. The new species represent the first non-marine Chersodromia reported from Southeast Asia and Papua New Guinea. Copyright © 2007 Magnolia Press.
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Causes of variability in diatom and Phaeocystis blooms in Belgian coastal waters between 1989 and 2003: A model study.
- Massive blooms of Phaeocystis colonies usually occur in the Belgian coastal zone (BCZ) between spring and summer diatom blooms but their relative magnitude varies between years. In order to understand this interannual variability, we used the biogeochemical MIRO model to explore the link between diatom and Phaeocystis blooms and changing nutrient loads and meteorological conditions over the last decade. For this application, MIRO was implemented in a simplified 3-box representation of the domain between the Baie de Seine and the BCZ. MIRO was run over the 1989–2003 period using actual photosynthetic active radiation (PAR), seawater temperature and riverine nutrient loads as forcing. The water mass residence time was calculated for each box based on a monthly water budget estimated from 1993–2003 water flow simulations of the three-dimensional hydrodynamical model COHSNS-3D. Overall MIRO simulations compare fairly well with nutrient and phytoplankton data collected in the central BCZ but indicate the importance of the hydrodynamical resolution frame for correctly describing the extremely high nutrient concentrations and biomass observed in the BCZ. Analysis of model results suggests that while interannual variability in diatom biomass depends on both meteorological conditions (light and temperature) and nutrient loads, Phaeocystis blooms are mainly controlled by nutrients. Further sensitivity tests with varying N and P loads suggest that only N reduction will result in significantly decreased Phaeocystis blooms without negative affects on diatoms, while P reduction will negatively affect diatoms. Moreover, Atlantic nutrient loads play such a great role in BCZ enrichment that reduction of Scheldt nutrient loads only is not sufficient to significantly decrease phytoplankton blooms in the BCZ. It is concluded that future nutrient reduction policies aimed to decrease Phaeocystis blooms in the BCZ without impacting diatoms should target the decrease of N loads in both the Seine and the Scheldt rivers.
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Dispersal mechanisms in amphipods: A case study of Jassa herdmani (Crustacea, Amphipoda) in the North Sea
- Jassa herdmani (Walker 1893), a tube-building amphipod typical of hard substrates, was found in large densities on shipwrecks from the Belgian part of the North Sea, in association with the hydrozoan Tubularia indivisa. In this area, shipwrecks only represent the source of hard substrates in an environment dominated by soft sediments. Nevertheless, the long-distance dispersal of Jassa species has never been investigated. Therefore, we tested the hypothesis of dispersal with currents, by investigating the behaviour of J. herdmani in the laboratory. Size distribution revealed that newly released juveniles (1 mm) predominated throughout the year and the lower frequency of the size class 1-3 mm indicates a high mortality or a dispersal at this life stage. Individuals of J. herdmani may initiate actively the transport by tidal or surface currents by swimming to the surface of the water or by floating at the surface, as suggested by behaviours noted in the laboratory. The reaction of the amphipods to a current was investigated in the laboratory. We could not detect any sexual function associated with drifting and there was also no preponderance of a size class in the drifting individuals. When testing different substrates, we observed a significant influence of the substrate type on the frequency at which drifting occurred: J. herdmani showed a better adherence on T. indivisa compared to other substrates. Finally, the amphipod showed also a preference for its host compared to other substrates, which suggests a possible detection mechanism. © 2007 Springer-Verlag.
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Isolation and characterization of polymorphic microsatellite markers in Galapagos prickly pear (Opuntia) cactus species: Primer note
- The Opuntia (prickly pear) genus contains over 200 species. Six of them are endemic to the Galapagos archipelago. Although these cacti are 'keystone' species of the Galapagos' semi-arid ecosystem, they have never been studied in detail. Because of their current threatened status and their important role in the ecosystem, we developed 16 microsatellite markers to study the population genetic structure of some of these species. These markers display a high level of polymorphism with numbers of alleles per locus ranging from six to 53. Results also revealed possible polyploidy in these cacti. © 2006 The Authors.
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Holocene sedimentary evolution and palaeocoastlines of the Lower Khuzestan plain (southwest Iran)
- The Holocene sequence of the Lower Khuzestan plain in southwest Iran has been investigated in the context of coastal evolution and relative sea-level change. A literature review about the coastal evolution of the Shatt-el Arab region with respect to relative sea-level changes is provided. The sedimentary succession in undisturbed hand-operated cores and temporary outcrops is described and facies are identified on the basis of lithology, sedimentary structures and macrofossils. Four main sedimentary environments are interpreted from the Holocene sedimentary record of the plain: brackish tidal flat, clastic coastal sabkha, brackish-freshwater marsh and fluvial plain. The study of the vertical and spatial distribution of sedimentary facies, their environmental interpretation especially with respect to the relationship to tide levels is done for five different zones in the plain. Chronological control is provided by radiocarbon dates. On the basis of this analysis, palaeogeographical reconstructions of the Lower Khuzestan plain, and the northern extension of the Persian Gulf were made for different points in time between 8000 and 450 cal BP. This study shows that during the early and middle Holocene, the Lower Khuzestan plain was a low-energy tidal embayment under estuarine conditions. During the initial sea-level rise of the early Holocene, the coastline rapidly transgressed across the shelf, and drowning of a major valley resulted in the development of extended tidal flats. Deceleration of sea-level rise after approximately 5500 cal BP, together with probably more arid conditions, allowed coastal sabkhas to extend widely and to aggrade while the position of the coastline remained relatively stable. Continued deceleration of sea-level rise initiated the progradation of the coastline from ca. 2500 cal BP. The effect of sediment supply by the rivers became more important than the effect of relative sea-level rise. The Karun megafan developed under a descelerating rate of sea-level rise, controlling the avulsive shifting of the rivers Karkheh and Jarrahi and their loci of sediment input. Moreover, in this study it is suggested that a Holocene RSL highstand, above the present-day sea level, as suggested by previous workers, did not occur. © 2007 Elsevier B.V. All rights reserved.
