Search publications of the members of the Royal Belgian institute of natural Sciences
-
The Late Gravettian Site of Kostënki 21 Layer III, Russia: a Chronocultural Reassessment Based on a New Interpretation of the Significance of Intra-site Spatial Patterning
-
Self-domestication or human control? The Upper Palaeolithic domestication of the wolf
-
Ancient RNA from Late Pleistocene permafrost and historical canids shows tissue-specific transcriptome survival
- While sequencing ancient DNA (aDNA) from archaeological material is now commonplace, very few attempts to sequence ancient transcriptomes have been made, even from typically stable deposition environments such as permafrost. This is presumably due to assumptions that RNA completely degrades relatively quickly, particularly when dealing with autolytic, nuclease-rich mammalian tissues. However, given the recent successes in sequencing ancient RNA (aRNA) from various sources including plants and animals, we suspect that these assumptions may be incorrect or exaggerated. To challenge the underlying dogma, we generated shotgun RNA data from sources that might normally be dismissed for such study. Here, we present aRNA data generated from two historical wolf skins, and permafrost-preserved liver tissue of a 14,300-year-old Pleistocene canid. Not only is the latter the oldest RNA ever to be sequenced, but it also shows evidence of biologically relevant tissue specificity and close similarity to equivalent data derived from modern-day control tissue. Other hallmarks of RNA sequencing (RNA-seq) data such as exon-exon junction presence and high endogenous ribosomal RNA (rRNA) content confirms our data’s authenticity. By performing independent technical library replicates using two high-throughput sequencing platforms, we show not only that aRNA can survive for extended periods in mammalian tissues but also that it has potential for tissue identification. aRNA also has possible further potential, such as identifying in vivo genome activity and adaptation, when sequenced using this technology.
-
'Zonnesponzen' uit het Krijt van Luik-Limburg na bijna 160 jaar opnieuw gevonden
-
Intermediate gastropod hosts of major feline cardiopulmonary nematodes in an area of wildcat and domestic cat sympatry in Greece
-
On the identity of Conus pennaceus sensu stricto Born, 1778, Conus pennaceus forma episcopus (Hwass in Bruguiére, 1792), Conus magnificus Reeve, 1843 and Conus quasimagnificus da Motta, 1982 from the coastal waters of Egypt, Red Sea
-
Regional Priorities 2024 – update by the ICG-RP
- Document OTSOPA 24/02/02 (+Add.1-4) presented by the Co-convenors of ICG-RP (Belgium and France) at the Meeting of the Working Group on Operational, Technical and Scientific Questions Concerning Counter Pollution Activities (OTSOPA), Lisbon, Portugal, 22-24 May 2024, 5 pp.
-
Aerial Surveillance Expert Assessment Panel.
- Anon., 2024b. Aerial Surveillance Expert Assessment Panel. Document OTSOPA 24/05/01 Rev.1 (+ Add.1) presented by the Chair of the EAP-AS and the Secretariat at the Meeting of the Working Group on Operational, Technical and Scientific Questions Concerning Counter Pollution Activities (OTSOPA), Lisbon, Portugal, 22-24 May 2024, 3 pp.
-
Update from the MARPOL Annex VI Working Groups on strategic and operational issues (MAVI SOWG) and on technical issues (MAVI TWG)
-
Regional Priorities 2024 – update by the ICG-RP
-
Draft report on Tour d’Horizon 2023 and multi-annual TdH trends
-
Update from the MARPOL Annex VI Working Groups on strategic and operational issues (MAVI SOWG) and on technical issues (MAVI-TWG)
-
Findings as well as technical and operational implications of MARPOL Annex VI related issues at international level
-
Tour d’Horizon 2024, 09-13 SEPTEMBER 2024. Mission Report.
-
Identifying EU-listed Aquatic Invasive Species by DNA-barcoding using currently available sequence data
-
A mitogenomic approach to resolve the phylogeny of Afrotropical hoverflies (Syrphidae)
-
Description of two new small cryptic species of Terebridae from the Philippines and Papua New Guinea
-
The Duplicaria duplicata (Linnaeus, 1758)-complex (Gastropoda: Terebridae): Part V. The description of six new species
-
An annotated list of the Panamic taxa of Terebridae, with the description of a new species of Hastula
-
Notes on Terebridae Part II: Revision of the taxa Terebra anilis and fenestrata, with the description of two new species and the introduction of a new name
