Search publications of the members of the Royal Belgian institute of natural Sciences
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On some Indo-West Pacific species of Favartia sensu stricto (Gastropoda: Muricidae: Muricopsinae) with the description of three new species from the Indian Ocean and comments on related species
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Living conditions of captive baboons and their trade in ancient Egypt revealed through palaeopathological and demographic data
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Paleogenomic insights into cat domestication in ancient Egypt
- Situated at the crossroads of Africa, Asia and Europe, Egypt is considered one of the two potential cradles of cat domestication. Zooarchaeological evidence points to cat-human relationships as old as the 4th millennium BC. Later, cats were object of a cult dedicated to the goddess Bastet, and from the 1st millennium BC until the 4th century AD were mummified as votive offerings. Previous ancient DNA (aDNA) investigation showed that cats in ancient Egypt possessed two maternal lineages commonly found in modern domestic cats. Haplotype-C, which spread all over the Old World since Classical Antiquity from Northern Africa, and haplotype-A, which was associated with an earlier cat dispersal from the Levant during the Neolithic. DNA from cat mummies represents a key tool to unravel the role of Egypt as a possible independent centre of domestication, even though the retrieval of aDNA from mummified tissues has greatly been challenged by DNA preservation. Here, we show the preliminary results of aDNA analyses conducted on more than 50 Egyptian cat mummies from Beni Hassan and Gourna (Luxor), dated to the Greco-Roman period. By comparing different methods (e.g. single and double stranded genomic library construction), we provide an extensive case for screening DNA preservation in arid regions, and within different tissues, such as hair, claws, and petrous bones. We also explore the potential to gain novel insights on the dispersal of domestic cats from Egypt in Classical Antiquity through mitochondrial and genome-wide data.
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Insights on zoonotic diseases in cat domestication through ancient pathogen genomics
- The growing interest in paleopathogenomics and microbial archaeology of the last decade offered the chance to investigate the complex relationships between human hosts and microorganisms, making it possible to identify and characterize the etiologic agents of epidemics in our past [1]. The spread of zoonotic disease in human history was associated with the progressively closer contact with domestic animals[1]. However, the role of animal hosts in the emergence of zoonoses has been only marginally explored, the main focus of paleopathogenomic research being primarily on human hosts. Due to their pivotal role as pest control agents and their long-standing relationship with humans, cats are hosts of several zoonotic diseases representing a threat for human health. Here we present the results of the metagenomic screening for ancient pathogen identification on more than 100 ancient cat remains from different geographic locations (Europe, North Africa and Southwest Asia) spanning several millennia from prehistory to historical times. Various bone substrates (teeth, postcranial skeletal elements, and petrous bones) were analyzed via shotgun sequencing and metagenomic screening with Kraken 2[2] and MetaPhlAn 4[3]. By applying strict authentication guidelines, we provide a framework of ancient microbial DNA preservation in cat remains across time and space, discussing the identification of potential zoonotic candidates. The identification of zoonotic microorganisms in ancient animals represents a yet overlooked field of research, which may offer unprecedented insights into zoonotic epidemics and inter-species transmissibility.
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Paleogenomics of European wild and domestic cats
- Zooarchaeological and genetic evidence from the last two decades demonstrated that domestic cats originated from the North African and Near Eastern wildcat, Felis silvestris lybica. The commensal relationship between humans and cats most likely started 11 thousand years ago (kya) in the Neolithic Levant. More recently, ancient mitochondrial DNA (mtDNA) evidence suggested that domestic cats spread to Southeast Europe as early as 4400 BC, however their dispersal to the rest of Europe is controversial due to the paucity of data. Furthermore, complex scenarios of admixture between domestic and wild populations (e.g., the European wildcat F. s. silvestris and the Asian wildcat F. s. ornata) may have taken place across time, thus leaving a mtDNA-based reconstruction unsatisfactory. Here we show the preliminary results of our paleogenomic investigation from more than 150 cat remains from European archaeological sites dated from 15 kya to the 18th century AD, with a peculiar focus on the Mediterranean area. By screening the samples for endogenous cat DNA content, we provide a framework of ancient DNA preservation in cat remains across time and space. Furthermore, by generating complete mtDNAs and low-coverage nuclear genome data (ranging from 0.2- to 1.4-fold), we were able to refine the chronology of cat dispersal in the Mediterranean region, and to address questions around potential admixture patterns between wild and domestic cat populations. Our paleogenomic dataset lay the foundations for future and more in-depth analyses aimed at understanding the factors determining the evolutionary success of the domestic cat.
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Using ancient DNA to identify Bos primigenius in ancient cattle remains from Belgium
- Aurochs (Bos primigenius) are the wild ancestors of the domesticated taurine cattle (Bos taurus). During the Holocene, populations of aurochs gradually declined until their extinction at the turn of the 17th century. DNA data suggest that domestic cattle in Europe descended from Near East aurochs that were domesticated and brought to Europe by the first farmers during the Neolithic period. Hybridization occurred more recently in Europe between domestic cattle and local wild aurochs. Most aurochs can be distinguished from domestic cattle osteometrically, but large-sized domestic cattle may be misidentified as aurochs. Based on mitochondrial DNA, most European aurochs differ from domestic cattle (haplogroup “P” versus “T”). With the aim to provide new data on the former distribution of aurochs in Europe, we used mitochondrial DNA to identify large bovid bones attributed to aurochs and dating from Epipaleolithic to medieval times. DNA was extracted from the bones of 11 specimens from Belgium in an ancient DNA lab. Shotgun DNA sequencing provided raw reads comprising 0.02-10% of endogenous DNA. For three samples, reads covered 74-98% of the bovid mitochondrial genome and enabled the identification of one aurochs from the Bronze Age (haplogroup “P”) and two cows from Roman and medieval times (haplogroup “T”). Among the other samples (covering 2-18% of the mitogenome), three Roman specimens could be assigned to the haplogroup “T” based on a few diagnostic positions. This study provides the first mitogenomic data for a Belgian aurochs and contributes to the identification of exceptionally large Roman bovid remains.
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Exploring the Morphometric Characteristics of sheep breeds in the Ancient Near East
- The multidisciplinary project Evosheep studies the origin and evolution of ancient sheep breeds by means of archaeozoology, geometric morphometrics, genetics, iconography and epigraphy in the ancient Near East from the six millennium BCE. This approach conducted on ancient breeds is completed by work on modern breeds from Near and Middle East and East Africa to provide new biometric and genetic reference records more adequate than European races for further scientific research. This osteological reference offers a measurement baseline for comparison with the archaeological data to explore the diversity patterns of the different domestic sheep populations across the Ancient Near East. This paper compares the diversity of sizes, allometry, and body parts proportions of sheep from archaeological assemblages using the Log Size Index, the Log Shape Ratio, and multivariate analyses. This study will focus on Chalcolithic and Bronze Age sites from the Levant, Mesopotamia, Iran, and South Caucasus.
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How ‘Shaun the sheep’ looked like in the past? Evolution and archaeological diversity of sheep morphotypes in Southwest Asia
- Sheep husbandry has played a leading role in the economy of human societies over the last 10 millennia. While archaeological research focused on the study of early husbandry practices, little is known about the emergence and morphological evolution of sheep breeds. To what extent ancient sheep bones could help to understand this change and identifying the factors behind it? To answer these questions, we used 3D geometric morphometrics methods on 260 astragalus of modern and archaeological sheep from Southwest Asia and East Africa. Ancient sheep bones have been recovered from forty-two archaeological sites covering seven millennia of economic and cultural transformations, between the 8th and the 1st millennium BC. We have explored morphometric diversity of sheep bones, first according to biotic and abiotic factors on well-documented modern osteological collections and second, according to environmental, chronological and socio-economical contexts on modern and ancient bones. Our first results indicate significant differences in the variation of astragalus shape between modern and archaeological sheep in correlation with ecological and chronocultural factors.
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Paleogenetic analysis of cat mummies from ancient Egypt
- Situated at the crossroads of Africa, Asia and Europe, Egypt is considered one of the two potential cradles of cat domestication (Felis catus). Here, cats most likely developed their relationship with humans with a dual role. In fact, on the one hand Egyptian iconography depicts cats as skilled hunters, and on the other hand as companion animals, quietly sitting under the chairs of noble people and as guardians of the deceased (Yoyotte & Vernus, 2005). Egyptian cats were object of a cult dedicated to the goddess Bastet, and from the 1st millennium BC until the 4th century AD were mummified as votive offerings. Previous ancient DNA (aDNA) investigation (Ottoni 2017) showed that cats in ancient Egypt possessed two maternal lineages commonly found in modern domestic cats. Haplotype-C, which spread all over the Old World since Classical Antiquity from Northern Africa, and haplotype-A, which was associated with an earlier cat dispersal from the Levant during the Neolithic. DNA from cat mummies represents a key tool to unravel the role of Egypt as a possible independent centre of domestication, despite the fact that the retrieval of aDNA from mummified tissues has greatly been challenged by DNA preservation. Here, we show the preliminary results of aDNA analyses conducted on more than 50 Egyptian cat mummies from Beni Hassan and Gourna (Luxor), dated to the Greco-Roman period. By comparing different methods (e.g. single and double stranded genomic library construction), we provide an extensive case for screening DNA preservation in arid regions, and within different tissues, such as hair, claws, and petrous bones. We also explore the potential to gain novel insights on the dispersal of domestic cats from Egypt in Classical Antiquity through mitochondrial and genome-wide data.
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Spatial scales affecting termite diversity in tropical lowland rainforest: a case study in southeast Cameroon
- Termites play a prominent role in the decomposer food web as 'ecosystem engineers'. However, the effect of spatial scales on termite assemblages has, to date, been poorly studied. Here, we evaluate the contribution of two spatial levels [transects (100 m x 2 m) and sections within transects (5 m x 2 m)] on the local termite assemblage of a continuous mixed secondary lowland rainforest in southeast Cameroon. Standardized 100 m x 2 m transects were run in five vegetation types to cover more of the heterogeneity of this forest. The additive partitioning of the species richness of all species, soil- and wood-feeder species was statistically tested with sample-based randomization. The average species richness per transect (61) was the highest ever recorded. The section scale significantly influenced the observed species richness of all groups. This suggests that physical complexity and heterogeneity exist on a small scale within each transect and create a variety of microhabitats for different termites. Still, soil-feeder species were less influenced by this scale than wood-feeder species. Although different vegetation types were sampled, transect scale did not enhance observed termite species diversity. Overall, as a wide variety and high density of microhabitats is inherent to large primary or regenerating forests, conservation programmes should focus on interconnecting large tropical rainforest blocks in Central Africa to maintain biodiversity hot spots of termites and optimal ecosystem functioning.
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Anatomy, Relationships, and Paleobiology of Cambaytherium (Mammalia, Perissodactylamorpha, Anthracobunia) from the lower Eocene of western India
- The anatomy of Cambaytherium, a primitive, perissodactyl-like mammal from the lower Eocene Cambay Shale Formation of Gujarat, India, is described in detail on the basis of more than 350 specimens that represent almost the entire dentition and the skeleton. Cambaytherium combines plesiomorphic traits typical of archaic ungulates such as phenacodontids with derived traits characteristic of early perissodactyls. Cambaytherium was a subcursorial animal better adapted for running than phenacodontids but less specialized than early perissodactyls. The cheek teeth are bunodont with large upper molar conules, not lophodont as in early perissodactyls; like perissodactyls, however, the lower molars have twinned metaconids and m3 has an extended hypoconulid lobe. A steep wear gradient with heavy wear in the middle of the tooth row suggests an abrasive herbivorous diet. Three species of Cambaytherium are recognized: C. thewissi (∼23 kg), C. gracilis (∼10 kg), and C. marinus (∼99 kg). Body masses were estimated from tooth size and long bone dimensions. Biostratigraphic and isotopic evidence indicates an age of ca. 54.5 Ma for the Cambay Shale vertebrate fauna, the oldest Cenozoic continental vertebrate assemblage from India, near or prior to the initial collision with Asia. Cambaytheriidae (also including Nakusia and Perissobune) and Anthracobunidae are sister taxa, constituting the clade Anthracobunia, which is sister to Perissodactyla. We unite them in a new higher taxon, Perissodactylamorpha. The antiquity and occurrence of Cambaytherium—the most primitive known perissodactylamorph—in India near or before its collision with Asia suggest that Perissodactyla evolved during the Paleocene on the Indian Plate or in peripheral areas of southern or southwestern Asia.
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“Marginal” landscapes: human activity, vulnerability and resilience in the Western Taurus mountains (South West Turkey)
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Man’s best friends
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Milk production at Bademağacı (SW Turkey) during the Early Neolithic: Archaeozoological data and residue analysis combined
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Faunal Remains of Bademağacı: Campaigns 1993-2005
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Een potje boven het vuur
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Étude anthropologique du squelette du puits I
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An interdisciplinary study around the reliquary of the late cardinal Jacques de Vitry
- The reliquary of Jacques de Vitry, a prominent clergyman and theologian in the early 13th century, has experienced several transfers over the last centuries, which seriously question the attribution of the remains to the late Cardinal. Uncertainty about the year of his birth poses an additional question regarding his age at death in 1240. The reliquary, located in the Saint Marie d’Oigines church, Belgium, was reopened in 2015 for an interdisciplinary study around his relics as well as the Treasure of Oignies, a remarkable cultural heritage notably built from Jacques de Vitry’s donation. Anthropological, isotopic and genetic analyses were performed independently on the remains found in the reliquary. Results of the analyses provided evidence that the likelihood that these remains are those of Jacques de Vitry is very high: the remains belong to the same human male individual and the historical tradition about his age is confirmed. In addition, a separate relic (left tibia) was analysed and found to match with the remains of the reliquary (right tibia). The unique Jacques de Vitry’s mitre, made of parchment, was sampled non-destructively and the extracted parchment collagen was analysed by a proteomic method in order to determine the animal species. The results showed that, surprisingly, not all parts of the mitre were made from the same species. All together, these findings are expected to fertilize knowledge carried by historical tradition around the relics of Jacques de Vitry and his related cultural heritage.
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Children at Work: Looking for Evidence in Past Societies
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Throwing Activities Among Neolithic Populations from the Meuse River Basin (Belgium, 4500–2500 BC) with a Focus on Adolescents.
- The anterior band of the medial collateral ligament (MCL) is an important stabilizer for valgus stress at the elbow. When practised intensively and repetitively, activities using overhead throwing motions can injure this ligament and its insertion. If these activities occur when the epiphyses are not yet fully fused, traction forces can result in bony detachments in the area of the MCL insertion. This study was based on commingled graves found in 16 Middle and Late Neolithic caves from Belgium. We recorded the presence of MCL lesions on 196 humeri and studied the relation between lesions, siding, and robusticity. 5.1% of the humeri displayed MCL lesions, which affected only the right robust humeri. Our results suggest a social division in throwing activities in Belgium during the Neolithic. They also suggest that throwing practice started from a young age, which invites us to re-examine the role of teenagers in prehistoric societies.
