The aim of this study was to assess the performance of selected destruction methods for the determination of heavy metals (Cd, Cu, Pb and Zn) in woodlouse (Oniscus asellus). A vigorous total analysis involving microwave destruction with HE HCl and HNO3 (method 1) was used as a reference method. Consistently low values for the dry ashing method may indicate incomplete dissolution of the elements and/or losses through volatilisation. Method 3 (concentrated HNO3) that frequently is used in literature, produced erroneous values for Cd, Cu and Pb. Results were consistent with the microwave digestion, provided H2O2 was used during digestion (method 4, HNO3/H2O2). Method 5 (HNO3/HClO4, one destruction step) yielded low recoveries when only one destruction step was applied. Applying two destruction steps (method 6) resulted in values consistent with the microwave method, but was at the expense of reproducibility and rendered the method more lengthy and laborious. Because of the very good performance combined with speed and simplicity, destruction with HNO3/H2O2 (method 4) emerged as the most convenient method.