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Xiaohua Su, Dapeng Jin, Chunying Li, Hua Yue, Yabin Pu, Yuehui Ma, and Weijun Guan (2011)

Establishment of Big Bone chicken fibroblast cell bank and study of its biological characteristics


A fibroblast bank of Big Bone chicken was established using tissue adherent culture method. This cell bank included 29 embryo samples, had stocks of 147 cryogenically-preserved vials each containing 2 similar to 3x10(6) cells, and met all the cell line quality control standards established by the American Type Culture Collection (ATCC). The cells cultured in vitro showed the typical morphology of fibroblasts. The growth curve assumed the ``S'' shape, and the cell population doubling time (PDT) was about 30h. Cell viability was 98.1\% before cryopreservation and 96.6\% after recovery. All tests for microbial contamination were negative. The isoenzyme pattern was of species specificity. The frequency of diploid cells was 91\%. The transfection efficiency of three fluorescent protein genes fluctuated between 10.6\% and 26.5\%. These results showed that the cells cultured in vitro grew well and had stable genetic properties. This research thus does not only preserve the poultry genetic resources of Big Bone chicken at the cell level, but also opens new ways for preserving important genetic resources of endangered animals in the form of somatic cells.

Big Bone chicken; fibroblast cell bank; biological characteristics
  • ISSN: 0777-6276

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium


Annales de la Société malacologique de Belgique
​Annales de la Société royale malacologique et zoologique de Belgique
Annales de la Société Royale Zoologique de Belgique
Belgian Journal of Zoology