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You are here: Home / Associated publications / Belgian Journal of Zoology / Bibliographic References / Expression of collapsin response mediator protein-4 (CRMP-4) in plastic brain areas of adult songbird brain

Ning Zhu, Shaoju Zeng, Xinwen Zhang, and Mingxue Zuo (2008)

Expression of collapsin response mediator protein-4 (CRMP-4) in plastic brain areas of adult songbird brain


Collapsin response mediator protein-4 (CRMP-4), a member of CRMP family, is involved in neuronal differentiation and regulation of synaptic rearrangement. Neurite growth and axonal reorganization still continue in the adult songbird brain. It is probable for CRMP-4 to play an important role during these processes. To address this issue, we first obtained CRMP-4 cDNA from the brain of songbird (Lonchura striata) by use of RT-PCR and 3' rapid amplification of cDNA ends (3'RACE). We then examined the expression of CRMP-4 mRNA and protein in some plastic brain regions, including the hippocampus, song control nuclei and the cerebellum. Double immunohistochemistry was employed to identify the phenotypes of CRMP-4 expressing cells. The results indicated that: 1) a cloned fragment of CRMP-4 cDNA (798bp) showed high similarity with those comparable cDNA of reported species, confirming the evolutionary conservation of CRMP-4 sequence; 2) both CRMP-4 mRNA and protein were found to be dominantly located in the hippocampus, song control nuclei and the cerebellum among the whole brain; 3) some CRMP-4 positive cells were also labeled by the antibody for HuD (immature neurons), but not for NeuN (mature neurons), and GFAP (astrocytes). The presence of CRMP-4 in the plastic regions suggests that CRMP-4 might be concerned with the neural plasticity of songbird brain.

CRMP-4; RT-PCR; in situ hybridization; immunohistochemistry; songbird brain
  • ISSN: 0777-6276

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium


Annales de la Société malacologique de Belgique
​Annales de la Société royale malacologique et zoologique de Belgique
Annales de la Société Royale Zoologique de Belgique
Belgian Journal of Zoology