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You are here: Home / Associated publications / Belgian Journal of Zoology / Bibliographic References / The use of demecolcine for enucleation of bovine oocytes

Xiang Li, Yong Zhang, Song Hua, Jian Shu, Zhi Zhang, and Jun Cao (2007)

The use of demecolcine for enucleation of bovine oocytes


Nuclear transfer requires removing all genetic materials associated with the chromosomes of recipient oocyte. This Study was designed to further explore the pharmacological role of Demecolcine (DM) on assisting enucteation in animal somatic cell nuclear transfer. The in vitro matured bovine oocytes were incubated with DM at different concentration, or at a fixed DM concentration for additional different hours, then the oocytes extrusion cones rate (ECR) were determined in the inverted microscope. The highest ECR (61.90\%) was measured from the treatment with 0.5gg/mL demecolcine. The time-dependent manner of the development of the extrusion cones, in 2hr groups were significantly higher (P < 0.05) than in Ohr, 0.5hr, 1 hr and 2.5hr groups. The highest ECR were found in vitro matured 18hr groups (73.86\%), which was significantly higher (P < 0.05) than that observed in 14hr, 16hr groups, and 20hr groups. However, the Granualar cell existence during maturation can influence on PR and embryos development rate. The in vitro matured 18hr bovine oocytes with granualar cell were added to DM, which were significantly higher (P < 0.05) than that of control groups. Meanwhile, we evaluated the effects of DM on the cleavage rate of in vitro matured oocytes. The results showed that the IVF medium with or without DM, the lVF embryos rate of cleavage, of blastocysts, and average cell number of blastocysts between the two groups were not significantly different from each other. This simple, chemically assisted method to remove maternal chromosomes makes it possible to produce a large number of nuclear-transferred eggs and to efficiently produce cloned bovines.

bovine; assisted-enucleation; demecolcine; nuclear transfer; oocyte
  • ISSN: 0777-6276

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium


Annales de la Société malacologique de Belgique
​Annales de la Société royale malacologique et zoologique de Belgique
Annales de la Société Royale Zoologique de Belgique
Belgian Journal of Zoology