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Inproceedings Reference Towards a sustainable management of pond diversity at the landscape level (Pondscape)
Located in Library / RBINS Staff Publications
Unpublished Reference Towards an Integrated Lake Basin Management Plan for Lake Manyara, Tanzania, using a tiered multistakeholder approach.
Located in Library / RBINS Staff Publications 2016
Article Reference Towards an operational sediment transport model for optimizing dredging works in the Belgian Coastal Zone (southern North Sea).
Located in Library / RBINS Staff Publications
Unpublished Reference Towards answering the “so what” question in marine renewables environmental impact assessment
Located in Library / RBINS Staff Publications 2017
Inproceedings Reference Towards Estimating the Biogeochemical Footprint of an Offshore Windfarm
Located in Library / RBINS Staff Publications 2020
Inproceedings Reference Towards investigating the effect of fining and hardening of marine coastal areas on ecosystem functioning in the Belgian part of the North Sea (BPNS)
Sediments and the inhabiting macrofauna play an important role in the functioning of coastal marine environments. Human activities in these environments result in two obvious pressures on the sediment: hardening from the installation of offshore wind farms (OWF) and fining in the vicinity of sand extraction sites and in OWF. This study is part of the FaCE-It project and aims at understanding the impacts of those pressures on biogeochemical cycling and on the food web structure. In order to understand benthic ecosystem functioning in different types of sediment, we will measure nutrient fluxes in closed-core incubations sampled along a gradient from fine to coarse sediments, including sediments subjected to fining. Bio- and physical irrigation will be quantified by adding a bromide tracer to the overlying water and following the decreasing concentration over time. With those data, we will estimate the total flux of degradable organic carbon deposited at the sediment surface, its degradation rate and transformation and assess the effect of bioturbation and bio irrigation on benthic ecosystem functioning. Along the same sediment gradient, we will study the macrobenthic food web using stable isotope analysis. Furthermore, the overall food web will be investigated at 3 stations characterized by fine sediment, coarse sediment and an OWF to compare the food web complexity in areas with and without OWFs. These data will finally be used to build a quantitative food web using linear inverse modelling (LIM). To constrain this model, individual respiration rate measurements and pulse chase experiments will be conducted. This combined approach will allow assessing the effect of fining and hardening of marine coastal areas on important ecological processes on the scale of the BPNS.
Located in Library / RBINS Staff Publications 2016
Inproceedings Reference Towards less invasive methods to inventory and monitor wildlife in the Congo Basin
Located in Library / RBINS Staff Publications 2017
Inproceedings Reference Towards the DNA barcoding of the Congolese riverine fish fauna
Located in Library / RBINS Staff Publications
Article Reference Trace elements analysis of the historical skeletal remains of Sainte-Croix-en-Jarez (16.18 th C., France). 9th Congress EAA, Copenhagen, August 24-27 1994
Located in Library / RBINS Staff Publications
Inproceedings Reference Tracing ancient DNA of Foraminifera in tsunami deposits (GEN-EX)
1. Background Tsunami deposits provide information on the long-term frequency-magnitude patterns of events, which may not be covered by the historical and instrumental record. Such information is crucial for the assessment of coastal hazards and mitigation measures against the loss of life and assets. In order to identify tsunami deposits in the coastal sedimentary record and to infer tsunami characteristics, a wide range of proxies has been established based on studies of recent tsunami deposits. Microfossils (e.g. foraminifera, ostracods, diatoms) are often used to recognize tsunami deposits, and to differentiate them from those of other processes. In terms of foraminifera, tsunami deposits mostly contain allochthonous associations dominated by benthic intertidal to inner shelf taxa. Specimens may originate from outer shelf to bathyal depths; even planktonic forms may occur. Furthermore, changes in test numbers, taphonomy, size or adult/juvenile ratios compared to background sedimentation are common (Pilarczyk et al., 2014; Engel et al., 2016). However, dissolution of microfossils often prevent identification and diminish their value as a proxy (Yawsangratt et al., 2012). 2. Study goals and concept To address the problem of post-depositional alteration of microfossil associations in tsunami deposits, high-throughput metagenomic sequencing techniques are applied by the GEN-EX project to identify marine organisms in onshore sand layers based on their DNA remains. Metagenomics (or environmental genomics) is related to sequencing DNA directly from the environmental samples, where the genetic material may have been preserved in sedimentary records covering tens of thousands of years. Metagenomics is an emerging technique in environmental research and is used to characterize the diversity of bacterial communities but also higher organisms such as animals, plants and fungi of recent and ancient origin in a variety of settings, including ice, lake sediments, soils, cave deposits, and various types of surface waters. Metagenomics can also be used to detect cryptic diversity, ultimately providing more accurate estimates of biodiversity (Pedersen et al., 2015). Among the broad range of organisms, foraminifera (single-celled protists) show a water depth-related zonation in subtidal environments, and are the first to have been identified successfully in palaeo- tsunami deposits by their DNA (SzczuciĔski et al., 2016). The main objectives of GEN-EX include: quantifying the relationship between water depth and the distribution of different foraminiferal taxa where known tsunami deposits are present, using a comparative classic micropalaeontological and metagenomic approach; assessing the potential (based on both approaches) for identifying key indicator species in tsunami deposits in different coastal settings; and establishing how metagenomic approaches can contribute to the differentiation between storm and tsunami deposits. 3. DNA extraction DNA will be analysed in two types of material – modern extant foraminifera and sediments (tsunami deposits and adjacent layers). DNA extracted from single foraminiferal specimens will be followed by whole genome amplification to obtain sufficient DNA concentrations. Either part of the nuclear 18S rRNA region or the mitochondrial genome (mtDNA) will be amplified, before high-throughput sequencing of the amplicons. Sequences will be edited and aligned, and their identity verified by BLAST (Altschul et al., 1990) searches in Genbank and the Forambarcoding project ( A project-specific database of 18S and mtDNA data of the identified recent foraminifera will be constructed. Sampling of tsunami deposits and DNA extraction follows the protocol of SzczuciĔski et al. (2016). Suitable primers will be developed from our reference database of recent foraminifera to amplify overlapping short fragments of 18S or mtDNA of the target species. Amplicon concentration will be quantified and prepared for high-throughput sequencing. Sequence data will be analysed with different bioinformatics pipelines (e.g. QIIME), including quality control, removal of barcodes and adaptors, identification and removal of chimeric and redundant sequences, and comparisons with our own and open access databases of 18S data for defining Operational Taxonomic Units with 95% and 97% similarity cut-offs. 4. Study area One of the study areas, where the eDNA approach is applied, are the Shetland Islands, exposed to the mega-tsunami triggered by the early Holocene Storegga submarine slide off the coast of Norway. Sediment run-up of more than 25 m left a distinct landward-thinning sand layer with an erosive lower contact, large rip-up clasts, fining-upward sequences and marine diatoms in near-shore lakes and coastal peat lowlands. In addition to sediments associated with the Storegga tsunami, two younger tsunami deposits dated to c. 5 and 1.5 ka (Bondevik et al., 2005) are investigated. Sampling for the planned foraminiferal analyses and eDNA extraction of the deposits and their source area, comprising along the beach and subtidal area to the central shelf area is scheduled for the second half of March 2018. 5. Acknowledgements Funding is kindly provided by a BELSPO BRAIN-be pioneer grant (BR/175/PI/GEN-EX). 6. References Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J., 1990. Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410. Bondevik, S., Mangerud, J., Dawson, S., Dawson, A. & Lohne, Ø., 2005. Evidence for three North Sea tsunamis at the Shetland Islands between 8000 and 1500 years ago. Quaternary Science Reviews, 24, 1757–1775. Engel, M., Oetjen, J., May, S.M. & Brückner, H., 2016. Tsunami deposits of the Caribbean – Towards an improved coastal hazard assessment. Earth-Science Reviews, 163, 260–296. Pedersen, M.W., Overballe-Petersen, S., Ermini, L., Sarkissian, C.D., Haile, J., Hellstrom, M., Spens, J., Thomsen, P.F., Bohmann, K., Cappellini, E., Bærholm Schnell, I., Wales, N.A., Carøe, C., Campos, P.F., Schmidt, A.M.Z., Gilbert, M.T.P., Hansen, A.J., Orlando, L. & Willerslev, E., 2015. Ancient and modern environmental DNA. Philosophical Transactions of the Royal Society B, 370, 20130383. Pilarczyk, J.E., Dura, T., Horton, B.P., Engelhart, S.E., Kemp, A.C. & Sawai, Y., 2014. Microfossils in coastal environments as indicators of paleo-earthquakes, tsunamis and storms. Palaeogeography, Palaeoclimatology, Palaeoecology, 413, 144–157. SzczuciĔski, W., Pawłowska, J., Lejzerowicz, F., Nishimura, Y., KokociĔski, M., Majewski, W., Nakamura, Y. & Pawlowski, J., 2016. Ancient sedimentary DNA reveals past tsunami deposits. Marine Geology, 381, 29–33. Yawsangratt, S., SzczuciĔski, W., Chaimanee, N., Chatprasert, S., Majewski, W. & Lorenc, S., 2012. Evidence of probable paleotsunami deposits on Kho Khao Island, Phang Nga Province, Thailand. Natural Hazards, 63, 151–163.
Located in Library / RBINS Staff Publications 2018