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ParasiteBlitz: Adaptation of the BioBlitz concept to parasitology
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A BioBlitz is a rapid and intensive survey of a specific geographic area that brings together experts and often lay participants to assess biodiversity, typically of macrobiota that are easily observed and identifiable on-site. This concept has become popular across taxonomic fields, attracting interest globally to increase knowledge of local biodiversity. Inspired by the success of the approach, we undertook a ‘ParasiteBlitz’ at an unexplored locality (Stono Preserve, Charleston, South Carolina, USA) to determine its feasibility for parasites, whose assessment of diversity is largely neglected worldwide. We assembled a team of parasitologists with complementary expertise. Over 12 days in April 2023, we intensively screened fishes and aquatic invertebrates for parasites, and sampled sediment and water for environmental DNA (eDNA) metabarcoding from four aquatic habitats: wetland, freshwater pond, brackish impoundment, and tidal creek. We incorporated assistance from non-parasitologists and students. Details on methodologies and results are provided in individual papers in this Special IssueCollection. Traditional methods revealed the presence of ca. 100 species of seven major metazoan parasite taxa and the eDNA survey yielded over 1,100 amplicon sequence variants identified as parasites, most with sequences unmatched in GenBank, and resulting in only a few species identified as named species in the one-year post-Blitz timeframe we imposed upon ourselves for identification. Limitations and challenges of the ParasiteBlitz are discussed, and our results support that this approach can be effective for rapid discovery of the dimensions of parasite assemblages in an understudied environment and contribute to parasitology knowledge.
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RBINS Staff Publications 2025
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Cichlid fishes are promising underutilised models to investigate helminth-microbiome interactions
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The """"Old Friends Hypothesis"""" suggests insufficient exposure to symbionts hinders immune development, contributing to increased immune-related diseases in the Global North. The microbiome is often the focus; helminths, potentially also offering health benefits, lack attention. Infection and effect of helminths are influenced and perhaps determined by microorganisms. Mechanisms behind parasite-microbiome interactions are poorly understood, despite implications on host health. These interactions are typically studied for single helminth species in laboratory animal models, overlooking helminth diversity. Reviewing research on relationships between helminth and microbial diversity, yielded 27 publications; most focused on human or other mammalian hosts, relying on natural exposure rather than experimental helminth inoculation. Only about half investigated host health outcomes. This understudied potential warrants considering additional candidate model systems. Given the high helminthiasis burden and species diversity of helminths, we propose seeking models in the Global South, where a considerable proportion of research on diversity aspects of helminth-microbiome interactions took place. Low availability of genomic resources for helminths in the Global South, however, necessitates more integrative helminthological research efforts. Given substantial similarities in immune systems, several fishes are models for human health/disease. More effort could be done to establish this for cichlids, whose representatives in the African Great Lakes provide a well-delineated, closed natural system relevant to human health in view of fish-borne zoonoses and other water-borne parasites. A good baseline exists for these cichlids' genomics, parasitology, and microbiology. We suggest exploring African Great Lake cichlids as model hosts for interactions between microbial diversity, helminth diversity, and host health.
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RBINS Staff Publications 2025
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Adaptive evolution of stress response genes in parasites aligns with host niche diversity
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Background Stress responses are key the survival of parasites and, consequently, also the evolutionary success of these organisms. Despite this importance, our understanding of the evolution of molecular pathways dealing with environmental stressors in parasitic animals remains limited. Here, we tested the link between adaptive evolution of parasite stress response genes and their ecological diversity and species richness. We comparatively investigated antioxidant, heat shock, osmoregulatory, and behaviour-related genes (foraging) in two model parasitic flatworm lineages with contrasting ecological diversity, Cichlidogyrus and Kapentagyrus (Platyhelminthes: Monopisthocotyla), through whole-genome sequencing of 11 species followed by in silico exon bait capture as well as phylogenetic and codon analyses. Results We assembled the sequences of 48 stress-related genes and report the first foraging (For) gene orthologs in flatworms. We found duplications of heat shock (Hsp) and oxidative stress genes in Cichlidogyrus compared to Kapentagyrus. We also observed positive selection patterns in genes related to mitochondrial protein import (Hsp) and behaviour (For) in species of Cichlidogyrus infecting East African cichlids—a host lineage under adaptive radiation. These patterns are consistent with a potential adaptation linked to a co-radiation of these parasites and their hosts. Additionally, the absence of cytochrome P450 and kappa and sigma-class glutathione S-transferases in monogenean flatworms is reported, genes considered essential for metazoan life. Conclusions This study potentially identifies the first molecular function linked to a flatworm radiation. Furthermore, the observed gene duplications and positive selection indicate the potentially important role of stress responses for the ecological adaptation of parasite species.
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RBINS Staff Publications 2025
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Geodiversiteit uit een archeologische opgraving in het oude stadscentrum van Antwerpen - bron van informatie over romeinse en middeleeuwse handel en gebruik.
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RBINS Staff Publications 2025
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Endocranial anatomy and phylogenetic position of the crocodylian Eosuchus lerichei from the late Paleocene of northwestern Europe and potential adaptations for transoceanic dispersal in gavialoids
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Eosuchus lerichei is a gavialoid crocodylian from late Paleocene marine deposits of northwestern Europe, known from a skull and lower jaws, as well as postcrania. Its sister taxon relationship with the approximately contemporaneous species Eosuchus minor from the east coast of the USA has been explained through transoceanic dispersal, indicating a capability for salt excretion that is absent in extant gavialoids. However, there is currently no anatomical evidence to support marine adaptation in extinct gavialoids. Furthermore, the placement of Eosuchus within Gavialoidea is labile, with some analyses supporting affinities with the Late Cretaceous to early Paleogene “thoracosaurs.” Here we present novel data on the internal and external anatomy of the skull of E. lerichei that enables a revised diagnosis, with 6 autapormorphies identified for the genus and 10 features that enable differentiation of the species from Eosuchus minor. Our phylogenetic analyses recover Eosuchus as an early diverging gavialid gavialoid that is not part of the “thoracosaur” group. In addition to thickened semi-circular canal walls of the endosseous labyrinth and paratympanic sinus reduction, we identify potential osteological correlates for salt glands in the internal surface of the prefrontal and lacrimal bones of E. lerichei. These salt glands potentially provide anatomical evidence for the capability of transoceanic dispersal within Eosuchus, and we also identify them in the Late Cretaceous “thoracosaur” Portugalosuchus. Given that the earliest diverging and stratigraphically oldest gavialoids either have evidence for a nasal salt gland and/or have been recovered from marine deposits, this suggests the capacity for salt excretion might be ancestral for Gavialoidea. Mapping osteological and geological evidence for marine adaptation onto a phylogeny indicates that there was probably more than one independent loss/reduction in the capacity for salt excretion in gavialoids.
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RBINS Staff Publications 2025
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Clumped isotope thermometry (Δ47) measurements in marine gastropods suggest equilibrium precipitation
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The clumped isotope paleothermometer (Δ47) has been used to reconstruct temperatures from various biogenic carbonate archives. Calibration studies demonstrate that some biogenic carbonates precipitate in Δ47 equilibrium and record growth temperatures accurately (e.g., many bivalve mollusks), while others appear to exhibit disequilibrium, or ‘vital’, effects and yield isotopically reconstructed temperatures that are biased (e.g., shallow-water corals). These studies have largely excluded marine gastropods, so it is not known whether they tend to precipitate their shells in or out of isotopic equilibrium. In this study, we present seasonal-scale δ18O and seasonally targeted Δ47 and Δ48 measurements from modern marine gastropods representing 8 genera and 10 species, reconstructing apparent growth temperatures and screening for equilibrium precipitation. We find that most marine gastropods appear to precipitate in Δ47 and Δ48 equilibrium and faithfully record environmental temperatures, making them suitable for Δ47-paleothermometry. A few gastropods (Caviturritella/Turritella sp., Campanile symbolicum, Megastraea undosa) appear to precipitate out of Δ47 equilibrium, though these disequilibrium signatures may partially be explained by differences between actual growth temperatures and instrumental calibration temperatures (Caviturritella/Turritella sp., M. undosa) or differences between inner and outer layer precipitation (C. symbolicum). We present new Δ47-temperature data for 2 Middle Eocene Campanile giganteum fossils collected from the Paris Basin and discuss how to interpret the results in the context of our modern samples. Finally, in conducting this calibration we demonstrate paired Δ47/Δ48 as an effective tool to screen for disequilibrium precipitation in marine gastropods.
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RBINS Staff Publications 2025 OA
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A European monsoon-like climate in a warmhouse world
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The middle Eocene warmhouse period (45 million years ago) featured atmospheric carbon dioxide concentrations equivalent to those projected under high future emission scenarios. Seasonal- to weather timescale climate reconstructions from this period can provide critical insight into the impact of Anthropogenic warming on intra-annual variability in temperature and precipitation. Here, we combine daily-scale reconstructions of the evolution of temperature and the water cycle in western Europe based on stable oxygen and clumped isotope analyses on the fastest-growing gastropod known in the fossil record: Campanile giganteum. Our dataset shows that the middle Eocene of western Europe featured monsoon-like conditions, with seawater temperatures of ˊ24 °C during mild and wet winters, 30 °C during hot and dry spring and autumn seasons, and ˊ28 °C during warm and comparatively wet summers. Coupled climate model simulations using the Community Earth System Model indicate these seasonal variations in temperature and precipitation were driven by shifting atmospheric and oceanic circulation regimes over Western Europe, with winds from different directions bringing distinct waters to the region and minimal wind during spring reducing cooling through diminished latent heat flux. Our results highlight that Europe may experience wetter summers with more frequent extreme rainfall events under future high emissions scenarios.
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RBINS Staff Publications 2025 OA
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Reduced contribution of sulfur to the mass extinction associated with the Chicxulub impact event
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The Chicxulub asteroid impact event at the Cretaceous-Paleogene (K-Pg) boundary ˊ66 Myr ago is widely considered responsible for the mass extinction event leading to the demise of the non-avian dinosaurs. Short-term cooling due to massive release of climate-active agents is hypothesized to have been crucial, with S-bearing gases originating from the target rock vaporization considered an important driving force. Yet, the magnitude of the S release remains poorly constrained. Here we empirically estimate the amount of impact-released S relying on the concentration of S and its isotopic composition within the impact structure and a set of terrestrial K-Pg boundary ejecta sites. The average value of 67 ± 39 Gt obtained is ˊ5-fold lower than previous numerical estimates. The lower mass of S-released may indicate a less prominent role for S emission leading to a milder impact winter with key implications for species survival during the first years following the impact.
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RBINS Staff Publications 2025 OA
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Deciphering the influence of evolutionary legacy and functional constraints on the patella: an example in modern rhinoceroses amongst perissodactyls
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In mammals, the patella is the biggest sesamoid bone of the skeleton and is of crucial importance in posture and locomotion, ensuring the role of a pulley for leg extensors while protecting and stabilizing the knee joint. Despite its central biomechanical role, the relation between the shape of the patella and functional factors, such as body mass or locomotor habit, in the light of evolutionary legacy are poorly known. Here, we propose a morphofunctional investigation of the shape variation of the patella among modern rhinoceroses and more generally among perissodactyls, this order of ungulates displaying a broad range of body plan, body mass and locomotor habits, to understand how the shape of this sesamoid bone varies between species and relatively to these functional factors. Our investigation, relying on three dimensional geometric morphometrics and comparative analyses, reveals that, within Rhinocerotidae and between the three perissodactyl families, the shape of the patella strongly follows the phylogenetic affinities rather than variations in body mass. The patellar shape is more conservative than initially expected both within and between rhinoceroses, equids and tapirs. The development of a medial angle, engendering a strong mediolateral asymmetry of the patella, appears convergent in rhinoceroses and equids, while tapirs retain a symmetric bone close to the plesiomorphic condition of the order. This asymmetric patella is likely associated with the presence of a “knee locking” mechanism in both equids and rhinos. The emergence of this condition may be related to a shared locomotor habit (transverse gallop) in both groups. Our investigation underlines unexcepted evolutionary constraints on the shape of a sesamoid bone usually considered as mostly driven by functional factors.
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RBINS Staff Publications 2024 OA
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Niche partitioning in the odontocete fauna from the Early Miocene Chilcatay Formation of Peru
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