Bacterial endosymbionts of the groups Wolbachia , Cardinium and Rickettsiaceae are well known for their diverse effects on their arthropod hosts, ranging from mutualistic relationships to reproductive phenotypes. Here, we analysed a unique system in which the dwarf spider Oedothorax gibbosus is co-infected with up to five different endosymbionts affiliated with Wolbachia , ‘Candidatus Tisiphia’ (formerly Torix group Rickettsia ), Cardinium and Rhabdochlamydia . Using short-read genome sequencing data, we show that the endosymbionts are heterogeneously distributed among O. gibbosus populations and are frequently found co-infecting spider individuals. To study this intricate host–endosymbiont system on a genome-resolved level, we used long-read sequencing to reconstruct closed genomes of the Wolbachia , ‘Ca. Tisiphia’ and Cardinium endosymbionts. We provide insights into the ecology and evolution of the endosymbionts and shed light on the interactions with their spider host. We detected high quantities of transposable elements in all endosymbiont genomes and provide evidence that ancestors of the Cardinium , ‘Ca. Tisiphia’ and Wolbachia endosymbionts have co-infected the same hosts in the past. Our findings contribute to broadening our knowledge about endosymbionts infecting one of the largest animal phyla on Earth and show the usefulness of transposable elements as an evolutionary ‘contact-tracing’ tool.
Located in
Library
/
RBINS Staff Publications 2023
Pike (Esox lucius) is a large freshwater species with a wide distribution in Eurasia and North America that has been exploited since prehistoric times as is shown by the skeletal remains found on numerous archaeological sites. The role the species played in the subsistence of inland human settlements can be documented by its proportion within the spectrum of exploited fish and by reconstructing the body lengths of the pike and the amount of meat they represent. Osteometrical data in the literature allow size and, sometimes, meat weight reconstructions on the basis of the measurements of isolated cranial bones, but little attention has been paid thus far to the use of vertebrae for such reconstructions. Using a series of 27 dry skeletons of modern pike, here, we provide regression equations that allow an accurate estimation of the body length and weight of the fish on the basis of each of the first five vertebrae. Analysis of the size variation of the height, width and length of all the vertebral centra along the vertebral column, through the construction of the so-called Global Rachidian Profile, showed that only the length measurements can be of potential use for a rather crude estimation of body length. Finally, a case study is provided, using a large assemblage of Early Neolithic pike vertebrae, to show the potential of the new osteometrical data.
Located in
Library
/
RBINS Staff Publications 2021
