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Article Reference Coding region SNP analysis to enhance dog mtDNA discrimination power in forensic casework
Located in Library / RBINS Staff Publications
Article Reference Coexisting cryptic species of the Litoditis marina complex (Nematoda) show differential resource use and have distinct microbiomes with high intraspecific variability
Differences in resource use or in tolerances to abiotic conditions are often invoked as potential mechanisms underlying the sympatric distribution of cryptic species. Additionally, the microbiome can provide physiological adaptations of the host to environmental conditions. We determined the intra- and interspecific variability of the microbiomes of three cryptic nematode species of the Litoditis marina species complex that co-occur, but show differences in abiotic tolerances. Roche 454 pyrosequencing of the microbial 16S rRNA gene revealed distinct bacterial communities characterized by a substantial diversity (85-513 OTUs) and many rare OTUs. The core microbiome of each species contained only very few OTUs (2-6), and four OTUs were identified as potentially generating tolerance to abiotic conditions. A controlled experiment in which nematodes from two cryptic species (Pm1 and Pm3) were fed with either an E. coli suspension or a bacterial mix was performed, and the 16S rRNA gene was sequenced using the MiSeq technology. OTU richness was 10-fold higher compared to the 454 data set and ranged between 1118 and 7864. This experiment confirmed the existence of species-specific microbiomes, a core microbiome with few OTUs, and high interindividual variability. The offered food source affected the bacterial community and illustrated different feeding behaviour between the cryptic species, with Pm3 exhibiting a higher degree of selective feeding than Pm1. Morphologically similar species belonging to the same feeding guild (bacterivores) can thus have substantial differences in their associated microbiomes and feeding strategy, which in turn may have important ramifications for biodiversity-ecosystem functioning relationships.
Located in Library / RBINS Staff Publications 2016
Article Reference Cold Code: The global initiative to DNA barcode amphibians and nonavian reptiles
Located in Library / RBINS Staff Publications
Article Reference Cold tolerance and freeze-induced glucose accumulation in three terrestrial slugs.
Located in Library / RBINS Staff Publications
Article Reference Colimarena, a new replacement name for the genus Colima Jocqué & Baert, 2005 (Araneae: Zodariidae)
Located in Library / RBINS Staff Publications 2021
Article Reference Collagen Extraction from Recent and Fossil Bones: Quantitative and Qualitative Aspects
Type I collagen is the major protein in bones. The mineral matrix protects collagen from denaturation, thus permitting the recovery of large collagen peptides from fossil bones thousands or millions of years old. Collagen peptides are more or less denatured in fossil bones, with diagenetic alteration being the major cause of such denaturation. Classical extraction methods alter the remaining large collagen peptides by extensive solubilization. A method is described here that used shorter collagen solubilization times. Resulting collagen yields are favourably compared with classical methods. The size of the large peptide (10 kDa) fraction improves considerably. Combined with a particular concentration step, the use of this shorter solubilization technique should be useful for collagen analyses that necessitate large peptides, as in the case of palaeoimmunology. © 1995 Academic Press. All rights reserved.
Located in Library / RBINS Staff Publications
Article Reference Collagen stable isotopes provide insights into the end of the mammoth steppe in the central East European plains during the Epigravettian
Higher δ15N values in bone collagen of mammoth (Mammuthus primigenius) compared with coeval large herbivores is a classic trait of the mammoth steppe. An exception applies to the Epigravettian site of Mezhyrich (ca. 18–17.4 ka cal BP) in the central East European plains, where mammoth bones have δ15N values equivalent to or in a lower range than those of horse specimens (Equus sp.). We expanded our preliminary dataset to a larger sampling size of mammoth, other large herbivores, and carnivores from contemporaneous and nearby sites of Buzhanka 2, Eliseevichi, and Yudinovo. The unusual low mammoth δ15N values were confirmed at Buzhanka 2 and for some specimens from Eliseevichi, while most individuals from Yudinovo displayed the expected high δ15N values, meaning similar to those of the large canids. The possibility of a contrast in migration pattern is not supported since the δ34S values, a marker of mobility, do not correlate with the δ15N values of mammoth bone collagen. No clear chronological tendency could be revealed, at least not at the scale of radiocarbon dating. The low range in δ15N values is likely to reflect a change in the specific niche of the mammoth in the southern part of its distribution.
Located in Library / RBINS Staff Publications 2018
Article Reference Collecting and processing living, non-marine Ostracods
Located in Library / RBINS Staff Publications 2016
Webpublished Reference Collecting information and identification of Oscarella from Cabo Frio, Brazil (Porifera, Homoscleromorpha, Oscarellidae)
Located in Library / RBINS Staff Publications 2022 OA
Article Reference Colonization and diversification by the Chydorus sphaericus complex in the East African sky islands
Located in Library / RBINS Staff Publications