The detection of parasite infections in past populations has classically relied on microscopic analysis of sediment samples and coprolites. In recent years, additional methods have been integrated into paleoparasitology such as enzyme-linked immunosorbent assay (ELISA) and ancient DNA (aDNA). The aim of this study was to evaluate a multimethod approach for paleoparasitology using microscopy, ELISA, and sedimentary ancient DNA (sedaDNA) with a parasite-specific targeted capture approach and high-throughput sequencing. Using 26 samples dating from c. 6400 BCE to 1500 CE that were previously analyzed with microscopy and ELISA, we aimed to more accurately detect and reconstruct parasite diversity in the Roman Empire and compare this diversity to earlier and later time periods to explore temporal changes in parasite diversity. Microscopy was found to be the most effective technique for identifying the eggs of helminths, with 8 taxa identified. ELISA was the most sensitive for detecting protozoa that cause diarrhea (notably Giardia duodenalis). Parasite DNA was recovered from 9 samples, with no parasite DNA recovered from any pre-Roman sites. Sedimentary DNA analysis identified whipworm at a site where only roundworm was visible on microscopy, and also revealed that the whipworm eggs at another site came from two different species (Trichuris trichiura and Trichuris muris). Our results show that a multimethod approach provides the most comprehensive reconstruction of parasite diversity in past populations. In the pre-Roman period, taxonomic diversity included a mixed spectrum of zoonotic parasites, together with whipworm, which is spread by ineffective sanitation. We see a marked change during the Roman and medieval periods with an increasing dominance of parasites transmitted by ineffective sanitation, especially roundworm, whipworm and protozoa that cause diarrheal illness.
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RBINS Staff Publications 2025
The European yew (Taxus baccata L.) is a long-lived conifer of ecological, cultural, and historical importance across Eurasia. Despite its remarkable resilience, wide distribution, and symbolic importance, the species has experienced a long-term decline due to a complex interplay of climatic fluctuations, megafaunal extinctions, human exploitation, and insufficient regeneration. Recent studies in palaeoecology, archaeology, dendroecology, and conservation have revealed a species with greater ecological plasticity and a broader historical distribution than previously assumed. However, many fundamental questions remain unresolved, particularly regarding its biogeographical history, population dynamics, recruitment processes, and the drivers of its decline. This review stems from prior investigations of yew in the French Pyrenees and, more broadly, across Europe. These efforts led to a transdisciplinary seminar and opened a collaboration uniting >30 researchers across Eurasia. By synthesizing a wide array of data and perspectives, the article highlights key knowledge gaps and outlines emerging research priorities. These are organized thematically—past, present, and future—and include 25 questions on the species' ecological niche, life-history strategies, human interactions, genetic resilience, and conservation under global change. The article advocates for a shift towards integrative and long-term conservation strategies that embrace the historical legacies of yew populations, the general ecology of the species along with local ecological context dependence, and the urgency of future threats. By identifying pressing research needs, this review seeks to lay the foundation for new collaborative initiatives and to support evidence-based conservation of this emblematic yet understudied species.
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RBINS Staff Publications 2025
In the Roman world, as in many other cultures, ivory was perceived as raw material suitable for the carving of prestigious personal items. The types of Roman and Late Antique carved ivory objects that survived as well as their quantity and stylistic range is probably a result of their preservation contexts as well as their appreciation and sometimes ongoing use in later epochs. Regarding ivory combs decorated with bas-relief carvings, only nine specimens are ascribed to the Mediterranean and NW-Europe during Late Antiquity, all exclusively present biblical iconography. Information about their origin, object history and age is usually very limited. The first evidence that hunting scenes were still part of the ivory carving tradition in Late Antiquity is provided by a comb discovered in a mid-6th-century male inhumation burial at Deiningen, Nördlinger Ries. In addition to stylistic and technological comparison, SEM-imaging, ZooMS, ancient DNA (aDNA) sequencing and 87Sr/86Sr isotope ratios were applied to identify the species and possible origin. While size and structure point toward Loxodonta africana as a likely source, poor preservation of the material hindered more refined results by ZooMS or aDNA analyses and consequently a precise triangulation of the source area in combination with the strontium isotope ratios. Besides being a singular piece of craftsmanship, the ivory comb fits into an assumed network of production and distribution that spanned from Northern Africa to the Frankish realm north of the Alps.
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RBINS Staff Publications 2026
