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Medieval fish remains on the Newport ship identified by ZooMS collagen peptide mass fingerprinting
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Fish represent a key economic, social and ecological group of species that humans have exploited for tens of thousands of years. However, as many fish stocks are going into decline and with little known about the anthropogenic impacts on the health of the marine ecosystem pre-Industrial Revolution, understanding historical and archaeological exploitation of fish species is key to accurately modelling these changes. Here, we explore the potential of collagen peptide mass fingerprinting (also known as Zooarchaeology by Mass Spectrometry, or ZooMS) for identifying fish remains from the Medieval (fifteenth century) Newport ship wreck (Wales, UK), and in doing so we establish a set of biomarkers we consider useful in discriminating between European fish taxa through the inclusion of over 50 reference taxa. The archaeological results identified nine distinct taxonomic groups, dominated by ling (> 40%), and a substantial amount of cod (> 20%) and hake (~ 20%). The vast majority of samples (> 70%) were identified to species level, and the inability to identify the remaining taxonomic groups with confidence using ZooMS was due to the fact that the reference collection, despite being relatively large in comparison to those presented in mammalian studies, reflects only a small proportion of fish biodiversity from this region. Although the results clearly demonstrate the potential for ZooMS as a means of fish bone identification, the sheer number of different fish species that potentially make up ichthyoarchaeological assemblages leads to obvious requirements for the analysis on much greater numbers of modern reference specimens, or the acquisition of collagen sequences.
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RBINS Staff Publications 2022
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Mega-tsunami conglomerates and flank collapses of ocean island volcanoes
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RBINS Staff Publications 2018
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MEMOR: A database of archeological human remains collections from Flanders, Belgium
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Abstract The aim of this article is to describe a newly created open access database of archeological human remains collections from Flanders, Belgium. The MEMOR database (www.memor.be) was created to provide an overview of the current practices of loans, reburial, and the research potential of human skeletons from archeological sites currently stored in Flanders. In addition, the project aimed to provide a legal and ethical framework for the handling of human remains and was created around stakeholder involvement from anthropologists, geneticists, contract archeologists, the local, regional and national government agencies, local and national government, universities, and representatives of the major religions. The project has resulted in the creation of a rich database with many collections available for study. The database was created using the open-source Arches data management platform that is freely available for organizations worldwide to configure in accordance with their individual needs and without restrictions on its use. Each collection is linked to information about the excavation and the site the remains originate from, its size and time period. In addition, a research potential tab reveals whether any analyses were performed, and whether excavation notes are available with the assemblage. The database currently contains 742 collections, ranging in size from 1 to over 1000 individuals. New collections will continue to be added when new assemblages are excavated and studied. The database can also be expanded to include human remains collections from other regions and other material categories, such as archaeozoological collections.
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RBINS Staff Publications 2023
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Mesocyclops thermocyclopoides species-group: redefinition and content
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Within the genus Mesocyclops the thermocyclopoides species-group is defined on the basis of the presence of spiniform armature on the maxillar coxa. Examination of nineteen, mainly Palaeotropical species of Mesocyclops and nine representatives of the closely related genus Thermocyclops revealed, that the coxal part of the maxillar syncoxa of the species recently unified in the thermocyclopoides group is adorned with distinct rows of big spinules on its frontal surface. This character, with only one known exception in South Indian M. cf. papuensis, is absent in other species of Mesocyclops and all the Thermocyclops studied here. These species have no spinules on the maxillar coxa at all or very tiny scattered ones. © 1994 Kluwer Academic Publishers.
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Mesozoic marine reptile palaeobiogeography in response to drifting plates
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Metabarcoding free‐living marine nematodes using curated 18S and CO1 reference sequence databases for species‐level taxonomic assignments
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RBINS Staff Publications 2018
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Metallothionein gene and protein expression as a biomarker for metal pollution in natural gudgeon populations
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Gudgeons (Gobio gobio) from historically Cd and Zn contaminated sites in Flanders (Belgium) were found to be resistant to elevated Cd levels. In previous work, this increased resistance was largely explained by increased metallothionein (MT) expression. Recently, environmental cleanup efforts resulted in a significant decrease in Cd concentrations in the surface water. In this study, we evaluated the use of hepatic metal and metallothionein (MT) concentrations as biomarkers of metal exposure before and after the cleanup. Hepatic MT mRNA levels were determined after the environmental metal levels decreased in order to assess the applicability of MT gene expression as an environmental biomarker in natural fish populations. Our data show that both metallothionein protein and gene expression have the potential to be sensitive biomarkers for metal exposure. Significant correlations were found (a) among accumulated metal concentrations and both MT protein and mRNA levels, and (b) between MT protein and mRNA levels. However, our data illustrated that while MT protein and gene expression give a quantitative picture of metal load at a single time point, quantitative information in natural populations cannot always be obtained when different time points (including different years) are compared, since MT gene and protein expression are affected by many other factors in addition to the metal load. Furthermore, the result of the environmental cleanup was reflected in a decrease of hepatic Cd concentrations. Zn remained the most important factor determining MT concentrations. Finally, two differently sized MT mRNAs were amplified to test the hypothesis that 3′-UTR length can offer a protective advantage in conditions of environmental stress. Our data provided no evidence to support this hypothesis. In contrast, the ratio of the long mRNA variant relative to total MT mRNA was surprisingly constant, and independent of exposure history. © 2007 Elsevier B.V. All rights reserved.
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Metallothionein-like Proteins as Cost-efficient Medchansism to Reduce Fitness Effects of Cadmium Exposure
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RBINS Staff Publications
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Metapopulation processes affecting diversity and distribution of myrmecophiles associated with red wood ants.
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RBINS Staff Publications
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Method assessment and observer variation in age estimation: A comparative analysis of the Suchey-Brooks and the İşcan methods on an archaeological medieval population
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Age-at-death estimation methods have faced challenges in their applicability to past populations. This study aims to evaluate the assessment and observer variability of the Suchey-Brooks (SB) and İşcan methods within archaeological contexts. Our sample consisted of 400 individuals from the medieval cemetery of the Dunes Abbey in Koksijde, Belgium. The skeletal remains exhibited good preservation, especially for the İşcan methods, which showed higher sensitivity to preservation conditions. Intra- and inter-observer variation were investigated using Spearman correlation coefficients, Wilcoxon paired ranks test, and Cohen's kappa test. The Spearman coefficient revealed a strong positive correlation, with a moderate to almost excellent kappa coefficient for both intra- and inter-observer error. Overall, the SB method displayed better inter-observer agreement, while the İşcan method presented better intra-observer agreement. Applying these methods to younger individuals resulted in less variability. Certain descriptive phase categories, such as phase III for the SB method and phase 3 and 4 for the İşcan methods, seemed problematic. Only 188 sufficiently preserved individuals could be scored using both the SB and the İşcan methods. They were classified into age groups, and the methods assessment were evaluated using the same statistical approach. The Spearman correlation coefficient indicated consistent age group attribution between the two methods (rho = 0.92), with a moderate to almost excellent agreement (k = 0.45; wk = 0.91). Overall, the two methods mainly differ by only one age group, except for older adults. The SB method tended to assign older age groups compared to İşcan's method, leading to effective overestimation.
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RBINS Staff Publications 2023
