Skip to content. | Skip to navigation

Personal tools

 
 
 
 

 
                 
You are here: Home / Associated publications / Belgian Journal of Zoology / Bibliographic References / Distribution of proliferating cells and vasa-positive cells in the embryo of Macrostomum lignano (Rhabditophora, Platyhelminthes)

Maxime Willems, Marjolein Couvreur, Mieke Boone, Wouter Houthoofd and Tom Artois (2010)

Distribution of proliferating cells and vasa-positive cells in the embryo of Macrostomum lignano (Rhabditophora, Platyhelminthes)

BELGIAN JOURNAL OF ZOOLOGY, 140(S):149-153.

The neoblast stem cell system of flatworms is considered to be unique within the animal kingdom. How this stem cell system arises during embryonic development is intriguing. Therefore we performed bromodeoxyuridine labelling on late stage embryos of Macrostomum lignano to assess when the pattern of proliferating cells within the embryo is comparable to that of hatchlings. This pattern can be found in late embryonic stages ( stage 8). We also used the freeze cracking method to perform macvasa embryonic labelling. Macvasa is a somatic and germ line stem cell marker. We showed macvasa protein distribution during the whole embryonic development. In the macvasa-positive blastomeres the protein is localized around the nucleus in the putative chromatoid bodies. However, at a specific embryonic stage, it is also ubiquitously present in the cytoplasm of some blastomeres. We compare our data with what is known from Schmidtea polychroa of the expression of the vasa-like gene SpolvlgA and the protein distribution of the chromatoid body component Spoltud-1. The embryonic origin of the somatic stem cell system and the germ line is discussed.

Macrostomorpha; embryonic development; neoblasts; markers; germ line
11th International Symposium on Flatworm Biology, Diepenbeek, BELGIUM, JUL 26-31, 2009
BJZ

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Editor-in-Chief:
Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium

 



1863-1903
Annales de la Société malacologique de Belgique
 
1903-1923
​Annales de la Société royale malacologique et zoologique de Belgique
 
1923-1989
Annales de la Société Royale Zoologique de Belgique
 
1989-
Belgian Journal of Zoology