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You are here: Home / Associated publications / Belgian Journal of Zoology / Bibliographic References / Cloning and tissue distribution of the cyclic AMP generating peptide of the grey flesh fly Neobellieria bullata (Diptera : Sarcophagidae)

M Begum, N Macours, L Schoofs, MM Rahman, A De Loof and R Huybrechts (2003)

Cloning and tissue distribution of the cyclic AMP generating peptide of the grey flesh fly Neobellieria bullata (Diptera : Sarcophagidae)

BELGIAN JOURNAL OF ZOOLOGY, 133(2):151-156.

Originally, two forms of cyclic AMP Generating Peptide (cGP) were identified in whole body extracts of the flesh fly Neobellieria bullata. The long form, a 48-mer, stimulated cAMP production by Malpighian tubules of Manduca sexta. The short form, which had been discovered previously with an ovarian bioassay, turned out to be the 1-15 aa sequence of the 48-mer. We here report on the cloning, sequencing and expression profile of the cGP-gene in N. bullata. The full-length cDNA sequence, obtained by RT-PCR in combination with 5' and 3' RACE, encodes a single copy of the cGP precursor. No signal peptide is present in this precursor, which, compared to the mature peptide, is only extended at the N-terminus with an extra methionine residue. RT-PCR revealed that Neb-cGP is expressed in both larval and adult brain, testis and ovaries, flight muscles, Malpighian tubules, midgut, and fat body.

insect endocrinology; reproduction; neuropeptide; gonadotropin; testis
BJZ

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Editor-in-Chief:
Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium

 



1863-1903
Annales de la Société malacologique de Belgique
 
1903-1923
​Annales de la Société royale malacologique et zoologique de Belgique
 
1923-1989
Annales de la Société Royale Zoologique de Belgique
 
1989-
Belgian Journal of Zoology