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You are here: Home / Associated publications / Belgian Journal of Zoology / Bibliographic References / Elevated salinities may enhance the recovery of hydrated heat-shocked Artemia franciscana cysts (International Study on Artemia. LXV)

TJ Abatzopoulos, GV Triantaphyllidis, N Roedaki, AD Baxevanis, A Triantafyllidis and P Sorgeloos (2003)

Elevated salinities may enhance the recovery of hydrated heat-shocked Artemia franciscana cysts (International Study on Artemia. LXV)

BELGIAN JOURNAL OF ZOOLOGY, 133(2):103-109.

Hydrated encysted embryos of the crustacean Artemia franciscana Kellogg 1906 were incubated at 42degreesC and 44degreesC for 48 h at elevated salinities. They exhibited better recovery when incubation took place in a high salinity environment. The recovery and/or protection by salinity of A. franciscana encysted embryos depend on the severity of the heat shock. Cysts heat shocked at 44degreesC and different salinity media (35, 50 and 70 ppt), upon return to optimal conditions, gave similar hatching percentages of less than 5\%. When heat shock took place at 80 ppt, a significant increase in hatching was observed (i.e. from 5\% to 22\%). Concentration of glycerol and translocation of a major protein of about 26 kD (p26) in Artemia encysted embryos were studied to reveal the potential protective role of these two factors against elevated temperatures. Increased glycerol levels were scored when cysts were incubated in elevated salinities within the first 4 h and prior to heat shock treatment (i.e. a 38 \% and 49 \% increase at the salinities of 70 ppt and 80 ppt, respectively). SDS-PAGE of proteins extracted from control and heat-shocked cysts suggested that the intracellular translocation of p26 was enhanced as the salinity increased. It is proposed that salinity-dependent heat shock seems to be at least partly responsible for i) the significant increase of glycerol and ii) the degree of translocation of p26, a suspected molecular chaperone described previously. These two factors along with others may contribute to the better recovery of heat-shocked Artemia encysted embryos. This phenomenon, which may be attributed to increased thermostability of proteins, is reflected as different cyst hatching ability.

Artemia franciscana; salinity; heat shock; glycerol; p26
BJZ

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Editor-in-Chief:
Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium

 



1863-1903
Annales de la Société malacologique de Belgique
 
1903-1923
​Annales de la Société royale malacologique et zoologique de Belgique
 
1923-1989
Annales de la Société Royale Zoologique de Belgique
 
1989-
Belgian Journal of Zoology