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You are here: Home / Associated publications / Belgian Journal of Zoology / Bibliographic References / SEM and cytofluorimetric characterization of Dinocras cephalotes haemocytes (Plecoptera, Perlidae)

JMT de Figueroa, AM Fausto, R Fochetti, G Scapigliati, E Sezzi and M Mazzini (2002)

SEM and cytofluorimetric characterization of Dinocras cephalotes haemocytes (Plecoptera, Perlidae)

BELGIAN JOURNAL OF ZOOLOGY, 132(1):65-69.

Haemocytes of the stonefly Dinocras cephalotes have been characterized by light and scanning electron microscopy (SEM), adherence to a plastic surface, and phagocytosis of latex particles. Haemocytes appear to consist of at least two cell populations: granulocytes and plasmatocytes, which can be distinguished by morphological and functional criteria. Morphologically, granulocytes are rounded and vesiculated, while plasmatocytes have an irregular shape, with many filopodia, and a slightly vesiculated content. Culturing haemocytes ``in vitro'' resulted in the spreading onto the surface of an adherent population (plasmatocytes), and in a loosely attached population (granulocytes). Phagocytosis assay showed the capacity of D. cephalotes plasmatocytes to engulf exogenous particles. Haemocytes of adults and nymphs of D. cephalotes were also analysed by flow cytometry and compared with those of Perla grandis, another species of Perlidae. We observed a slight difference in haemocyte morphology among nymphs and adults, and a shift of cell populations was also detected.

haemocytes; stoneflies; plasmatocytes; granulocytes; phagocytosis; flow cytometry
BJZ

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Editor-in-Chief:
Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium

 



1863-1903
Annales de la Société malacologique de Belgique
 
1903-1923
​Annales de la Société royale malacologique et zoologique de Belgique
 
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Annales de la Société Royale Zoologique de Belgique
 
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Belgian Journal of Zoology