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You are here: Home / Associated publications / Belgian Journal of Zoology / Bibliographic References / Production of intestine-specific monoclonal antibody and interspecific cross-reaction in triclads and polyclads

Y Asano, A Yoshida, N Isozaki and S Ishida (2001)

Production of intestine-specific monoclonal antibody and interspecific cross-reaction in triclads and polyclads

BELGIAN JOURNAL OF ZOOLOGY, 131(S1):137-141.

We have produced a monoclonal antibody (mAb(2)2B6) specific to intestinal and epidermal cells of Dugesia japonica. This mAb(2)2B6 also reacted with the secretory granules of a kind of sub-epidermal gland cells, epithelial cells and gland cells in the pharynx. The results of the interspecific cross-reaction tests with four other freshwater triclads and four polyclads showed that this mAb(2)2B6 can be used as a marker, specific to only intestinal cells in both Planocera multitentaculata and Planocera reticulata of polyclads. Immuno-electron microscopical research revealed that the cytoplasm of phagocytic cells and the spherical large granules in granular clubs reacted strongly to this mAb in the intestine. Also, mAb positive material seemed to be absorbed by pinocytosis from phagocytic cells into the granular clubs. These observations support the theory that the granular clubs are protein reserve cells, not gland cells for intraluminal digestion.

intestine; monoclonal antibody; interspecific cross-reaction; Platyhelminthes
9th International Symposium on the Biology of the Turbellaria (ISBT), BARCELONA, SPAIN, JUN, 2000
BJZ

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Editor-in-Chief:
Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium

 



1863-1903
Annales de la Société malacologique de Belgique
 
1903-1923
​Annales de la Société royale malacologique et zoologique de Belgique
 
1923-1989
Annales de la Société Royale Zoologique de Belgique
 
1989-
Belgian Journal of Zoology