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You are here: Home / Associated publications / Belgian Journal of Zoology / Bibliographic References / Improved surface visualization of living avian blastoderm structures and neighbouring ooplasms by oocytal trypan-blue staining

M Callebaut, L Van Nassauw, F Harrisson and H Bortier (1998)

Improved surface visualization of living avian blastoderm structures and neighbouring ooplasms by oocytal trypan-blue staining

BELGIAN JOURNAL OF ZOOLOGY, 128(1):3-11.

By injection(s) of trypan-blue solution during late oogenesis (rapid growth period of the oocytes) into the mother quail we could improve the visibility of the structural details seen from the surface in living quail blastoderms when still in situ on their egg yolk balls. This technique of intraoocytal yolk staining by trypan blue allows us to observe, to focus on and to photograph much better the surface morphology of unincubated or shortly incubated blastoderms and their relationship with the neighbouring ooplasms. Indeed the difference in distribution and volume of the trypan-blue-stained yolk granules in the blastoderms and neighbouring ooplasms seems to prevent excessive reflexion by the deep part of the germ disc of the light penetrating through the superficial parts. This diminished scattering of light, greatly increases the contrast between the different regions. By this method we could visualize a higher number of RAUBER's sickles from the surface in living unincubated quail blastoderms.

trypan-blue-stained yolk; avian blastoderm; Rauber's sickle; ooplasm; Japanese quail (Coturnix coturnix japonica)
BJZ

ISSN 2295-0451 (online version)
ISSN 0777-6279 (printed version)
impact factor 2015: 0,87.

Editor-in-Chief:
Prof. Dr. Isa Schön
Royal Belgian Institute of Natural Sciences
Vautierstraat 29
1000 Brussels, Belgium

 



1863-1903
Annales de la Société malacologique de Belgique
 
1903-1923
​Annales de la Société royale malacologique et zoologique de Belgique
 
1923-1989
Annales de la Société Royale Zoologique de Belgique
 
1989-
Belgian Journal of Zoology